Literature DB >> 26192344

Site-directed spin labeling of 2'-amino groups in RNA with isoindoline nitroxides that are resistant to reduction.

Subham Saha1, Anil P Jagtap, Snorri Th Sigurdsson.   

Abstract

Two aromatic isothiocyanates, derived from isoindoline nitroxides, were synthesized and selectively reacted with 2'-amino groups in RNA. The spin labels displayed limited mobility in RNA, making them promising candidates for distance measurements by pulsed EPR. After conjugation to RNA, a tetraethyl isoindoline derivative showed significant stability under reducing conditions.

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Year:  2015        PMID： 26192344     DOI： 10.1039/c5cc05014f

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  7 in total

1.  Impact of spin label rigidity on extent and accuracy of distance information from PRE data.

Authors:  K A Schnorr; D B Gophane; C Helmling; E Cetiner; K Pasemann; B Fürtig; A Wacker; N S Qureshi; M Gränz; D Barthelmes; H R A Jonker; E Stirnal; S Th Sigurdsson; H Schwalbe
Journal:  J Biomol NMR       Date:  2017-05-12       Impact factor: 2.835

2.  2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis.

Authors:  Jack S Hardwick; Marius M Haugland; Afaf H El-Sagheer; Denis Ptchelkine; Frank R Beierlein; Andrew N Lane; Tom Brown; Janet E Lovett; Edward A Anderson
Journal:  Nucleic Acids Res       Date:  2020-04-06       Impact factor: 16.971

3.  Site-Directed Spin Labeling of RNA with a Gem-Diethylisoindoline Spin Label: PELDOR, Relaxation, and Reduction Stability.

Authors:  Christine Wuebben; Simon Blume; Dinar Abdullin; Dominik Brajtenbach; Florian Haege; Stephanie Kath-Schorr; Olav Schiemann
Journal:  Molecules       Date:  2019-12-06       Impact factor: 4.411

4.  Phosphoramidite building blocks with protected nitroxides for the synthesis of spin-labeled DNA and RNA.

Authors:  Timo Weinrich; Eva A Jaumann; Ute M Scheffer; Thomas F Prisner; Michael W Göbel
Journal:  Beilstein J Org Chem       Date:  2018-06-26       Impact factor: 2.883

5.  Isoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling through Cysteines and Alkyne-Bearing Noncanonical Amino Acids.

Authors:  Theresa Sophie Braun; Pia Widder; Uwe Osswald; Lina Groß; Lara Williams; Moritz Schmidt; Irina Helmle; Daniel Summerer; Malte Drescher
Journal:  Chembiochem       Date:  2019-12-06       Impact factor: 3.164

6.  Cu2+-based distance measurements by pulsed EPR provide distance constraints for DNA backbone conformations in solution.

Authors:  Shreya Ghosh; Matthew J Lawless; Hanna J Brubaker; Kevin Singewald; Michael R Kurpiewski; Linda Jen-Jacobson; Sunil Saxena
Journal:  Nucleic Acids Res       Date:  2020-05-21       Impact factor: 16.971

7.  Compaction of RNA Duplexes in the Cell*.

Authors:  Alberto Collauto; Sören von Bülow; Dnyaneshwar B Gophane; Subham Saha; Lukas S Stelzl; Gerhard Hummer; Snorri T Sigurdsson; Thomas F Prisner
Journal:  Angew Chem Int Ed Engl       Date:  2020-10-13       Impact factor: 15.336
  7 in total

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