Guang-Jian Lu1, Yu-Qian Dong1, Qun-Mei Zhang2, Wen-Yu Di3, Lu-Yang Jiao1, Qing-Zu Gao3, Chen-Guang Zhang4. 1. Clinical Laboratory, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China. 2. Blood Transfusion Room, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China. 3. Department of Pathology, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China. 4. Medical Inspection Institute, Xinxiang Medical University Xinxiang 453000, China.
Abstract
INTRODUCTION: MicroRNAs (miRNAs) play important roles in tumorigenesis. In this study, we investigated the role of miR-221 in the development and progression of clear cell renal cell carcinoma (ccRCC). METHODS: Quantitative real-time PCR (qRT-PCR) was used to measure the expression level of miR-221 in ccRCC tissues and cell lines. Then, we investigated the role of miR-221 to determine its potential roles on renal cancer cell proliferation, migration and invasion in vitro. A luciferase reporter assay was conducted to confirm the target gene of miR-221 and the results were validated in renal cancer cells. RESULTS: In the present study, we found that miR-221 was significantly increased in ccRCC tissues and cell lines. Knocked-down expression of miR-221 remarkably inhibited cell proliferation, migration and invasion of renal cancer cells. Moreover, at the molecular level, our results suggested that TIMP2 as a direct target of miR-221 through which miR-221 promoted tumor cell proliferation, migration and invasion. CONCLUSIONS: These findings suggested that miR-221 play an oncogenic role in the renal cancer cell proliferation, migration and invasion by directly inhibiting the tumor suppressor TIMP2, indicating miR-221 act as a potential new therapeutic target for the treatment of ccRCC.
INTRODUCTION: MicroRNAs (miRNAs) play important roles in tumorigenesis. In this study, we investigated the role of miR-221 in the development and progression of clear cell renal cell carcinoma (ccRCC). METHODS: Quantitative real-time PCR (qRT-PCR) was used to measure the expression level of miR-221 in ccRCC tissues and cell lines. Then, we investigated the role of miR-221 to determine its potential roles on renal cancer cell proliferation, migration and invasion in vitro. A luciferase reporter assay was conducted to confirm the target gene of miR-221 and the results were validated in renal cancer cells. RESULTS: In the present study, we found that miR-221 was significantly increased in ccRCC tissues and cell lines. Knocked-down expression of miR-221 remarkably inhibited cell proliferation, migration and invasion of renal cancer cells. Moreover, at the molecular level, our results suggested that TIMP2 as a direct target of miR-221 through which miR-221 promoted tumor cell proliferation, migration and invasion. CONCLUSIONS: These findings suggested that miR-221 play an oncogenic role in the renal cancer cell proliferation, migration and invasion by directly inhibiting the tumor suppressor TIMP2, indicating miR-221 act as a potential new therapeutic target for the treatment of ccRCC.
Authors: F Fornari; L Gramantieri; M Ferracin; A Veronese; S Sabbioni; G A Calin; G L Grazi; C Giovannini; C M Croce; L Bolondi; M Negrini Journal: Oncogene Date: 2008-06-02 Impact factor: 9.867
Authors: Vincenzo Petrozza; Antonio Carbone; Teresa Bellissimo; Natale Porta; Giovanni Palleschi; Antonio Luigi Pastore; Angelina Di Carlo; Carlo Della Rocca; Francesco Fazi Journal: Int J Mol Sci Date: 2015-12-08 Impact factor: 5.923