Gaochao Qian1, Wentao Jin1, Xiaojing Tian1, Zhixiang Ding1, Bingwei Shi1, Qi Zhang2. 1. Department of Clinical Laboratory, Changzhou TCM Hospital Affiliated to Nanjing TCM University Changzhou 213003, China. 2. Department of Cardiology, Changzhou TCM Hospital Affiliated to Nanjing TCM University Changzhou 213003, China.
Abstract
OBJECTIVE: To investigate the cytoprotective effects of high dose of α-galactosylceramide (α-GC) on the activation-induced CD4+ T and CD8+ T cell death. METHODS: Experimental autoimmune encephalomyelitis (EAE) was induced using adoptive transfer of MOGCD4+ cells treated using α-GC into recipient C57BL/6 mice while the MOGCD4+ cells treated using 0.5% polysorbate were set as vehicle group, based on which to investigate the effects of α-GC on activation induced CD4+ T cell death. Additionally, an EG7 tumor-bearing mice model is established using adoptive transfer of CD8+ T cells, based on which to investigate the effect of α-GC on the apoptosis of CD8+ T cells. RESULTS: A higher induction rate was noticed after adoptive transfer of MOGCD4+ cells treated using α-GC together with the severity of EAE compared with the conventional methods. Longer survival duration was noted in the green fluorescent protein (GFP) labeled MOGT in the α-GC group compared with the vehicle group (P < 0.05). Severe inflammatory cell infiltration and myelinoclasis was noted in the white matter of nervous system in the α-GC group. In the EG7 tumor model, more adoptive CD8+ T cells were survived in α-GC group compared with that of vehicle group. The growth of tumor mass was significantly inhibited in α-GC group. CONCLUSIONS: high dose of α-GC could be used as an adjuvant for inhibiting activation-induced CD4+ T and CD8+ T cell death. Our study could provide helpful information for the development of adoptive cell therapy with reduced programmed cell death.
OBJECTIVE: To investigate the cytoprotective effects of high dose of α-galactosylceramide (α-GC) on the activation-induced CD4+ T and CD8+ T cell death. METHODS: Experimental autoimmune encephalomyelitis (EAE) was induced using adoptive transfer of MOGCD4+ cells treated using α-GC into recipient C57BL/6 mice while the MOGCD4+ cells treated using 0.5% polysorbate were set as vehicle group, based on which to investigate the effects of α-GC on activation induced CD4+ T cell death. Additionally, an EG7 tumor-bearing mice model is established using adoptive transfer of CD8+ T cells, based on which to investigate the effect of α-GC on the apoptosis of CD8+ T cells. RESULTS: A higher induction rate was noticed after adoptive transfer of MOGCD4+ cells treated using α-GC together with the severity of EAE compared with the conventional methods. Longer survival duration was noted in the green fluorescent protein (GFP) labeled MOGT in the α-GC group compared with the vehicle group (P < 0.05). Severe inflammatory cell infiltration and myelinoclasis was noted in the white matter of nervous system in the α-GC group. In the EG7 tumor model, more adoptive CD8+ T cells were survived in α-GC group compared with that of vehicle group. The growth of tumor mass was significantly inhibited in α-GC group. CONCLUSIONS: high dose of α-GC could be used as an adjuvant for inhibiting activation-induced CD4+ T and CD8+ T cell death. Our study could provide helpful information for the development of adoptive cell therapy with reduced programmed cell death.
Entities:
Keywords:
T cells; activation-induced cell death; experimental autoimmune encephalomyelitis; α-galactosylceramide
Authors: Ian F Hermans; Jonathan D Silk; Uzi Gileadi; Mariolina Salio; Bini Mathew; Gerd Ritter; Richard Schmidt; Adrian L Harris; Lloyd Old; Vincenzo Cerundolo Journal: J Immunol Date: 2003-11-15 Impact factor: 5.422
Authors: Tomasz B Owczarek; Jarosław Suchanski; Bartosz Pula; Alicja M Kmiecik; Marek Chadalski; Aleksandra Jethon; Piotr Dziegiel; Maciej Ugorski Journal: PLoS One Date: 2013-12-31 Impact factor: 3.240