Hye-Yeon Choi1, Min-Ju Seo1, Kyung-Chul Shin1, Deok-Kun Oh2. 1. Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea. 2. Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea. deokkun@konkuk.ac.kr.
Abstract
OBJECTIVE: To improve the production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid (HODA) from α-linolenic acid in Stenotrophomonas nitritireducens. RESULTS: Cells of the bacterium were permeabilized with 1.25% (v/v) methanol. The optimal conditions for HODA production by permeabilized cells were pH 7, 35 °C, 5% (v/v) DMSO, 50 g cells l(-1), and 22.5 g α-linolenic acid l(-1). Under these conditions, permeabilized cells produced 16.4 g HODA l(-1) after 2 h, with a conversion yield of 73 % (w/w) and a volumetric productivity of 8.2 g l(-1) h(-1). These values were 153 and 230 % of the values for non-permeabilized cells CONCLUSIONS: This is the highest concentration and volumetric and specific productivities of HODA reported thus far.
OBJECTIVE: To improve the production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid (HODA) from α-linolenic acid in Stenotrophomonas nitritireducens. RESULTS: Cells of the bacterium were permeabilized with 1.25% (v/v) methanol. The optimal conditions for HODA production by permeabilized cells were pH 7, 35 °C, 5% (v/v) DMSO, 50 g cells l(-1), and 22.5 g α-linolenic acid l(-1). Under these conditions, permeabilized cells produced 16.4 g HODA l(-1) after 2 h, with a conversion yield of 73 % (w/w) and a volumetric productivity of 8.2 g l(-1) h(-1). These values were 153 and 230 % of the values for non-permeabilized cells CONCLUSIONS: This is the highest concentration and volumetric and specific productivities of HODA reported thus far.