Literature DB >> 26187776

A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities.

Anne W Thompson1, Matthew J Crow2, Brian Wadey2, Christina Arens3, Serdar Turkarslan3, Sergey Stolyar3, Nicholas Elliott4, Timothy W Petersen2, Ger van den Engh5, David A Stahl4, Nitin S Baliga6.   

Abstract

A high speed flow cytometric cell sorter was modified to maintain a controlled anaerobic environment. This technology enabled coupling of the precise high-throughput analytical and cell separation capabilities of flow cytometry to the assessment of cell viability of evolved lineages of obligate anaerobic organisms from cocultures.
Copyright © 2015. Published by Elsevier B.V.

Keywords:  Anaerobic microorganism; Controlled-environment sorting; Flow cytometry; Single cells

Mesh：

Year:  2015        PMID： 26187776     DOI： 10.1016/j.mimet.2015.07.009

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  4 in total

Review 1.  Methods for quantification of growth and productivity in anaerobic microbiology and biotechnology.

Authors:  Lisa-Maria Mauerhofer; Patricia Pappenreiter; Christian Paulik; Arne H Seifert; Sébastien Bernacchi; Simon K-M R Rittmann
Journal:  Folia Microbiol (Praha)       Date:  2018-11-16       Impact factor: 2.099

2.  Mechanism for microbial population collapse in a fluctuating resource environment.

Authors:  Serdar Turkarslan; Arjun V Raman; Anne W Thompson; Christina E Arens; Mark A Gillespie; Frederick von Netzer; Kristina L Hillesland; Sergey Stolyar; Adrian López García de Lomana; David J Reiss; Drew Gorman-Lewis; Grant M Zane; Jeffrey A Ranish; Judy D Wall; David A Stahl; Nitin S Baliga
Journal:  Mol Syst Biol       Date:  2017-03-20       Impact factor: 11.429

3.  Printing Microbial Dark Matter: Using Single Cell Dispensing and Genomics to Investigate the Patescibacteria/Candidate Phyla Radiation.

Authors:  Sandra Wiegand; Hang T Dam; Julian Riba; John Vollmers; Anne-Kristin Kaster
Journal:  Front Microbiol       Date:  2021-06-16       Impact factor: 5.640

4.  Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditions.

Authors:  Samuel Bellais; Mélanie Nehlich; Maryne Ania; Aurore Duquenoy; Wilfrid Mazier; Ger van den Engh; Jan Baijer; Nicole Simone Treichel; Thomas Clavel; Ilia Belotserkovsky; Vincent Thomas
Journal:  Microbiome       Date:  2022-02-03       Impact factor: 14.650
  4 in total

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