Caroline Lourenço de Lima1, Michella Soares Coelho2, Carine Royer2, Augusto Pereira Resende3, Gabriel Alvares Borges3, Jaqueline Rodrigues da Silva4, Angélica Amorim Amato2, Eliete Guerra3, Francisco de Assis Rocha Neves2, Ana Carolina Acevedo3. 1. Laboratory of Oral Histopathology, Department of Dentistry, Faculty of Health Sciences, University of Brasília, Brasília, Distrito Federal, Brazil; Laboratory of Molecular Pharmacology, Department of Pharmacy, Faculty of Health Sciences, University of Brasília, Brasília, Distrito Federal, Brazil. Electronic address: carollourenco@yahoo.com.br. 2. Laboratory of Molecular Pharmacology, Department of Pharmacy, Faculty of Health Sciences, University of Brasília, Brasília, Distrito Federal, Brazil. 3. Laboratory of Oral Histopathology, Department of Dentistry, Faculty of Health Sciences, University of Brasília, Brasília, Distrito Federal, Brazil. 4. Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasília, Brasília, Distrito Federal, Brazil.
Abstract
INTRODUCTION: Rosiglitazone (RSG) is a synthetic full agonist of transcription factor peroxisome proliferator activated receptor gamma. Previous studies have suggested an anti-inflammatory effect of RSG on lipopolysaccharide-induced pulp inflammation. However, its role in other cellular events related to pulp repair has not been investigated. Therefore, the aim of the present study was to evaluate the effect of RSG on human dental pulp cell viability, proliferation, migration, and osteoblastic/odontoblastic differentiation. METHODS: Cell proliferation was evaluated by [3H]-thymidine assay. Cell viability was assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay and by measuring the percentage of apoptotic cells by flow cytometry. Cell migration was estimated by scratch wound healing assay. Mineralization and cell differentiation were evaluated by alizarin red S staining and real-time polymerase chain reaction gene expression assay, respectively. RESULTS: RSG significantly decreased cell proliferation and did not have effect on cell viability, apoptosis/necrosis, or migration. Alizarin red S showed that RSG accelerated calcified nodule formation. Results of real-time polymerase chain reaction demonstrated that RSG upregulated osteopontin expression, whereas expression of dentin sialophosphoprotein, dentin matrix protein-1, and osteocalcin was not affected. CONCLUSIONS: These findings suggest that RSG decreases human dental pulp cell proliferation, while positively regulating osteopontin expression.
INTRODUCTION:Rosiglitazone (RSG) is a synthetic full agonist of transcription factor peroxisome proliferator activated receptor gamma. Previous studies have suggested an anti-inflammatory effect of RSG on lipopolysaccharide-induced pulp inflammation. However, its role in other cellular events related to pulp repair has not been investigated. Therefore, the aim of the present study was to evaluate the effect of RSG on human dental pulp cell viability, proliferation, migration, and osteoblastic/odontoblastic differentiation. METHODS: Cell proliferation was evaluated by [3H]-thymidine assay. Cell viability was assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay and by measuring the percentage of apoptotic cells by flow cytometry. Cell migration was estimated by scratch wound healing assay. Mineralization and cell differentiation were evaluated by alizarin red S staining and real-time polymerase chain reaction gene expression assay, respectively. RESULTS:RSG significantly decreased cell proliferation and did not have effect on cell viability, apoptosis/necrosis, or migration. Alizarin red S showed that RSG accelerated calcified nodule formation. Results of real-time polymerase chain reaction demonstrated that RSG upregulated osteopontin expression, whereas expression of dentin sialophosphoprotein, dentin matrix protein-1, and osteocalcin was not affected. CONCLUSIONS: These findings suggest that RSG decreases human dental pulp cell proliferation, while positively regulating osteopontin expression.
Authors: Caroline L de Lima; Bruna R Amorim; Carine Royer; Augusto P Resende; Maria F Borin; Francisco A R Neves; Ana Carolina Acevedo Journal: PPAR Res Date: 2021-03-18 Impact factor: 4.964