Literature DB >> 26187203

Rapid Amplification of cDNA Ends for RNA Transcript Sequencing in Staphylococcus.

Eric Miller1.   

Abstract

Rapid amplification of cDNA ends (RACE) is a technique that was developed to swiftly and efficiently amplify full-length RNA molecules in which the terminal ends have not been characterized. Current usage of this procedure has been more focused on sequencing and characterizing RNA 5' and 3' untranslated regions. Herein is described an adapted RACE protocol to amplify bacterial RNA transcripts.

Keywords:  Amplification of cDNA ends; RNA mapping; Transcriptional start site; cDNA amplification; mRNA sequencing

Mesh：

Substances：

Year:  2016        PMID： 26187203     DOI： 10.1007/7651_2015_282

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Regulation of the CRISPR-Associated Genes by Rv2837c (CnpB) via an Orn-Like Activity in Tuberculosis Complex Mycobacteria.

Authors:  Yang Zhang; Jun Yang; Guangchun Bai
Journal:  J Bacteriol       Date:  2018-03-26       Impact factor: 3.490

2.  Efficient Method for Molecular Characterization of the 5' and 3' Ends of the Dengue Virus Genome.

Authors:  Alicia Rosales-Munar; Diego Alejandro Alvarez-Diaz; Katherine Laiton-Donato; Dioselina Peláez-Carvajal; Jose A Usme-Ciro
Journal:  Viruses       Date:  2020-04-29       Impact factor: 5.048

3.  Staphylococcal enterotoxin-like X (SElX) is a unique superantigen with functional features of two major families of staphylococcal virulence factors.

Authors:  Ries J Langley; Yi Tian Ting; Fiona Clow; Paul G Young; Fiona J Radcliff; Jeong Min Choi; Richard P Sequeira; Silva Holtfreter; Heather Baker; John D Fraser
Journal:  PLoS Pathog       Date:  2017-09-07       Impact factor: 6.823
  3 in total

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