| Literature DB >> 26186723 |
Hiroo Yoshida1, Kazuhiro Kondo2, Hiroyuki Yamamoto3, Naoko Kageyama4, Shin-Ichi Ozawa5, Kazutaka Shimbo6, Takahiko Muramatsu7, Akira Imaizumi8, Toshimi Mizukoshi9, Junichi Masuda10, Daisuke Nakayama10, Yoshihiro Hayakawa10, Kyoko Watanabe10, Kazuo Mukaibatake10, Hiroshi Miyano1.
Abstract
The analysis of human plasma free amino acids is important for diagnosing the health of individuals, because their concentrations are known to vary with various diseases. The development of valid, reliable, and high-throughput analytical methods for amino acids analysis is an essential requirement in clinical applications. In the present study, we have developed an automated precolumn derivatization amino acid analytical method based on high-performance liquid chromatography/electrospray ionization mass spectrometry (so-called UF-Amino Station). This method enabled the separation of at least 38 types of physiological amino acids within 8min, and the interval time between injections was 12min. We also validated this method for 21 major types of free amino acids in human plasma samples. The results of the specificity, linearity, accuracy, repeatability, intermediate precision, reproducibility, limits of detections, lower limits of quantification, carry over, and sample solution stability were sufficient to allow for the measurement of amino acids in human plasma samples. Our developed method should be suitable for use in clinical fields.Entities:
Keywords: Automated precolumn derivatization; LC–MS; Plasma free amino acid analysis; UF-Amino Station; Validation
Mesh：
Substances：
Year: 2015 PMID： 26186723 DOI： 10.1016/j.jchromb.2015.05.029
Source DB: PubMed Journal: J Chromatogr B Analyt Technol Biomed Life Sci ISSN： 1570-0232 Impact factor: 3.205