Saeid Ghorbian1, Issa Jahanzad2, Gholam Reza Javadi1, Ebrahim Sakhinia3. 1. a Department of Biology, Science and Research Branch , Islamic Azad University , Tehran , Iran. 2. b Department of Pathology, Imam Khomeini Hospital Complex , Medical Sciences/University of Tehran , Iran. 3. c Biotechnology Research Center , Tabriz University of Medical Sciences , Iran.
Abstract
BACKGROUND: Although the analysis of molecular clonality rearrangements of the immunoglobulin light chains (IGK and IGL) is an alternative approach for diagnosis of B cell non-Hodgkin lymphomas (NHLs) using BIOMED-2 protocols, NHLs have not been extensively confirmed for Hodgkin lymphoma (HL) cases. We evaluated BIOMED-2 protocols in HL cases, which have been suggested previously as gold standard method for molecular clonality analysis on formalin fixed, paraffin-embedded (FFPE) tissue in NHL patients. METHODS: We recruited 50 consecutive FFPE tissues of HL samples to evaluate IGK and IGL clonality gene rearrangements using BIOMED-2 and Heteroduplex methods. RESULTS: Our findings revealed a total of 94% (47/50) positive clonality, which consisted of 70% (35/50) for IGK and 44% (22/50) for IGL. In three cases, clonality was not detected in any of the immunoglobulin gene segments. CONCLUSIONS: Analysis of clonality gene rearrangements in IGK and IGL genes using BIOMED-2 protocols could be implemented as a valuable method for improving clonality detection rate in HL cases and sensitivity (94%) and accuracy of HL diagnosis similar to that of the NHL samples will be increased.
BACKGROUND: Although the analysis of molecular clonality rearrangements of the immunoglobulin light chains (IGK and IGL) is an alternative approach for diagnosis of B cell non-Hodgkin lymphomas (NHLs) using BIOMED-2 protocols, NHLs have not been extensively confirmed for Hodgkin lymphoma (HL) cases. We evaluated BIOMED-2 protocols in HL cases, which have been suggested previously as gold standard method for molecular clonality analysis on formalin fixed, paraffin-embedded (FFPE) tissue in NHLpatients. METHODS: We recruited 50 consecutive FFPE tissues of HL samples to evaluate IGK and IGL clonality gene rearrangements using BIOMED-2 and Heteroduplex methods. RESULTS: Our findings revealed a total of 94% (47/50) positive clonality, which consisted of 70% (35/50) for IGK and 44% (22/50) for IGL. In three cases, clonality was not detected in any of the immunoglobulin gene segments. CONCLUSIONS: Analysis of clonality gene rearrangements in IGK and IGL genes using BIOMED-2 protocols could be implemented as a valuable method for improving clonality detection rate in HL cases and sensitivity (94%) and accuracy of HL diagnosis similar to that of the NHL samples will be increased.
Authors: Diede A G van Bladel; Michiel van den Brand; Jos Rijntjes; Samhita Pamidimarri Naga; Demi L C M Haacke; Jeroen A C W Luijks; Konnie M Hebeda; J Han J M van Krieken; Patricia J T A Groenen; Blanca Scheijen Journal: Mod Pathol Date: 2021-12-03 Impact factor: 8.209
Authors: Diede A G van Bladel; Wendy B C Stevens; Michiel van den Brand; Leonie I Kroeze; Patricia J T A Groenen; J Han J M van Krieken; Konnie M Hebeda; Blanca Scheijen Journal: Cancers (Basel) Date: 2022-06-30 Impact factor: 6.575