Vassilis L Tzounakas1, Hara T Georgatzakou1, Anastasios G Kriebardis2, Effie G Papageorgiou3, Konstantinos E Stamoulis4,5, Leontini E Foudoulaki-Paparizos5, Marianna H Antonelou1, Issidora S Papassideri1. 1. Department of Cell Biology and Biophysics, Faculty of Biology, NKUA. 2. Laboratory of Hematology and Transfusion Medicine, Department of Medical Laboratories, Faculty of Health and Caring Professions, Technological and Educational Institute of Athens, Athens. 3. Laboratory of Information Science and Biostatistics, Department of Medical Laboratories, Faculty of Health and Caring Professions, Technological and Educational Institute of Athens; and. 4. Hellenic National Blood Center, Acharnes, Athens, Greece. 5. Regional Blood Transfusion Center, "Agios Panteleimon" General Hospital of Nikea, Piraeus, Greece.
Abstract
BACKGROUND: Oxidative stress orchestrates a significant part of the red blood cell (RBC) storage lesion. Considering the tremendous interdonor variability observed in the "storability," namely, the capacity of RBCs to sustain the storage lesion, this study aimed at the elucidation of donor-specific factors that affect the redox homeostasis during the storage of RBCs in standard systems. STUDY DESIGN AND METHODS: The hematologic profile of regular blood donors (n = 78) was evaluated by biochemical analysis of 48 different variables, including in vivo hemolysis and plasma oxidant and antioxidant factors and statistical analysis of the results. The possible effect of the uric acid (UA) variable on RBC storability was investigated in leukoreduced CPD/SAGM RBC units (n = 8) collected from donors exhibiting high or low prestorage levels of UA, throughout the storage period. RESULTS: Among the hematologic variables examined in vivo, cluster analysis grouped the donors according to their serum UA levels. Plasma antioxidant capacity, iron indexes, and protein carbonylation represented covariants of UA factor. RBCs prepared by low- or high-UA donors exhibited significant differences between them in spheroechinocytosis, supernatant antioxidant activity, and other RBC storage lesion-associated variables. CONCLUSION: UA exhibits a storability biomarker potential. Intrinsic variability in plasma UA levels might be related to the interdonor variability observed in the storage capacity of RBCs. A model for the antioxidant effect of UA during the RBC storage is currently proposed.
BACKGROUND: Oxidative stress orchestrates a significant part of the red blood cell (RBC) storage lesion. Considering the tremendous interdonor variability observed in the "storability," namely, the capacity of RBCs to sustain the storage lesion, this study aimed at the elucidation of donor-specific factors that affect the redox homeostasis during the storage of RBCs in standard systems. STUDY DESIGN AND METHODS: The hematologic profile of regular blood donors (n = 78) was evaluated by biochemical analysis of 48 different variables, including in vivo hemolysis and plasma oxidant and antioxidant factors and statistical analysis of the results. The possible effect of the uric acid (UA) variable on RBC storability was investigated in leukoreduced CPD/SAGM RBC units (n = 8) collected from donors exhibiting high or low prestorage levels of UA, throughout the storage period. RESULTS: Among the hematologic variables examined in vivo, cluster analysis grouped the donors according to their serum UA levels. Plasma antioxidant capacity, iron indexes, and protein carbonylation represented covariants of UA factor. RBCs prepared by low- or high-UA donors exhibited significant differences between them in spheroechinocytosis, supernatant antioxidant activity, and other RBC storage lesion-associated variables. CONCLUSION:UA exhibits a storability biomarker potential. Intrinsic variability in plasma UA levels might be related to the interdonor variability observed in the storage capacity of RBCs. A model for the antioxidant effect of UA during the RBC storage is currently proposed.
Authors: Renata E Boehm; Sabrina N Do Nascimento; Carolina R Cohen; Solange Bandiera; Rianne R Pulcinelli; Almeri M Balsan; Nuryan S Fao; Caroline Peruzzi; Solange C Garcia; Leo Sekine; Tor G H Onsten; Rosane Gomez Journal: Blood Transfus Date: 2019-11-20 Impact factor: 3.443
Authors: Angelo D'Alessandro; Xiaoyun Fu; Julie A Reisz; Mars Stone; Steve Kleinman; James C Zimring; Michael Busch Journal: Transfusion Date: 2020-05-08 Impact factor: 3.157