Zakaria Jalal1, Louise Galmiche2, David Lebeaux3, Olivier Villemain1, Georgia Brugada1, Mehul Patel1, Jean-Marc Ghigo2, Christophe Beloin2, Younes Boudjemline4. 1. Centre de Référence Malformations Cardiaques Congénitales Complexes - M3C, Necker Hospital for Sick Children, Assistance Publique des Hôpitaux de Paris, Pediatric Cardiology, Paris, France. 2. Institut Pasteur, Unité de Génétique des Biofilms, Département de Microbiologie, Paris, France. 3. Hôpital Necker Enfants Malades, Laboratoire d'Anatomopathologie, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France. 4. Centre de Référence Malformations Cardiaques Congénitales Complexes - M3C, Necker Hospital for Sick Children, Assistance Publique des Hôpitaux de Paris, Pediatric Cardiology, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France. Electronic address: younes.boudjemline@nck.aphp.fr.
Abstract
BACKGROUND: Percutaneous pulmonary valve implantation (PPVI) using Melody valve made of bovine jugular vein is safe and effective. However, infective endocarditis has been reported for unclear reasons. We sought to assess the impact of valvular substrates on selective bacterial adhesion. METHODS: Three valved stents (Melody valve, homemade stents with bovine and porcine pericardium) were tested in-vitro for bacterial adhesion using Staphylococcus aureus and Streptococcus sanguinis strains. RESULTS: Bacterial adhesion was higher on bovine jugular venous wall for S. aureus and on Melody valvular leaflets for S. sanguinis in control groups and significantly increased in traumatized Melody valvular leaflets with both bacteria (traumatized vs non traumatized: p=0.05). Bacterial adhesion was lower on bovine pericardial leaflets. CONCLUSION: Selective adhesion of S. aureus and S. sanguinis pathogenic strains to Melody valve tissue was noted on healthy tissue and increased after implantation procedural steps.
BACKGROUND: Percutaneous pulmonary valve implantation (PPVI) using Melody valve made of bovine jugular vein is safe and effective. However, infective endocarditis has been reported for unclear reasons. We sought to assess the impact of valvular substrates on selective bacterial adhesion. METHODS: Three valved stents (Melody valve, homemade stents with bovine and porcine pericardium) were tested in-vitro for bacterial adhesion using Staphylococcus aureus and Streptococcus sanguinis strains. RESULTS: Bacterial adhesion was higher on bovine jugular venous wall for S. aureus and on Melody valvular leaflets for S. sanguinis in control groups and significantly increased in traumatized Melody valvular leaflets with both bacteria (traumatized vs non traumatized: p=0.05). Bacterial adhesion was lower on bovine pericardial leaflets. CONCLUSION: Selective adhesion of S. aureus and S. sanguinis pathogenic strains to Melody valve tissue was noted on healthy tissue and increased after implantation procedural steps.
Authors: Bart W Driesen; Evangeline G Warmerdam; Gert-Jan Sieswerda; Folkert J Meijboom; Mirella M C Molenschot; Pieter A Doevendans; Gregor J Krings; Arie P J van Dijk; Michiel Voskuil Journal: Curr Cardiol Rev Date: 2019
Authors: Clara Stammnitz; Dörte Huscher; Ulrike M M Bauer; Aleksandra Urban; Johannes Nordmeyer; Stephan Schubert; Joachim Photiadis; Felix Berger; Sabine Klaassen Journal: J Am Heart Assoc Date: 2022-02-18 Impact factor: 5.501
Authors: Mohammad Abdelghani; Martina Nassif; Nico A Blom; Martijn S Van Mourik; Bart Straver; David R Koolbergen; Jolanda Kluin; Jan G Tijssen; Barbara J M Mulder; Berto J Bouma; Robbert J de Winter Journal: J Am Heart Assoc Date: 2018-06-22 Impact factor: 5.501