Oriol Sibila1,2, Guillermo Suarez-Cuartin1,2, Ana Rodrigo-Troyano1,2, Thomas C Fardon3, Simon Finch3, Eder Freddy Mateus2,4, Laia Garcia-Bellmunt1,2, Diego Castillo1,2, Silvia Vidal2,4, Ferran Sanchez-Reus2,5, Marcos I Restrepo6,7, James D Chalmers3. 1. Respiratory Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain. 2. Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain. 3. Tayside Respiratory Research Group, University of Dundee, Dundee, UK. 4. Laboratory of Experimental Immunology, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain. 5. Microbiology Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain. 6. Critical Care Department, South Texas Veterans Health Care System. 7. Medicine Division, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Abstract
BACKGROUND AND OBJECTIVE: Secreted mucins play a key role in antibacterial defence in the airway, but have not previously been characterized in non-cystic fibrosis (CF) bronchiectasis patients. We aim to investigate the relationship between secreted mucins levels and the presence of bacterial colonization due to potentially pathogenic microorganisms (PPM) in the airways of stable bronchiectasis patients. METHODS: Clinically stable bronchiectasis patients were studied prospectively at two centres. Patients with other pulmonary conditions were excluded. Spontaneous sputum was subject to bacterial culture, and secreted mucins (MUC2, MUC5AC and MUC5B) were measured in sputum supernatants by ELISA. RESULTS: A total of 50 patients were included. PPM were identified from sputum samples in 30 (60%), with Pseudomonas aeruginosa (n = 10) and Haemophilus influenzae (n = 10) as the most common PPM. There were no baseline differences among airway colonized and non-colonized patients. Patients with airways colonized by PPM presented higher levels of airway MUC2. No differences in MUC5AC levels were found among groups, whereas MUC5B levels were undetectable. Patients with P. aeruginosa colonization expressed the highest levels of MUC2. High levels of MUC2 and MUC5AC are also correlated with disease severity using the Bronchiectasis Severity Index. CONCLUSIONS: Airway MUC2 levels were higher in bronchiectasis patients colonized with PPM compared with those without airway colonization, especially in patients with P. aeruginosa. These findings suggest that airway-secreted mucins levels may play a role in the pathogenesis of airway infection in non-CF bronchiectasis.
BACKGROUND AND OBJECTIVE: Secreted mucins play a key role in antibacterial defence in the airway, but have not previously been characterized in non-cystic fibrosis (CF) bronchiectasispatients. We aim to investigate the relationship between secreted mucins levels and the presence of bacterial colonization due to potentially pathogenic microorganisms (PPM) in the airways of stable bronchiectasispatients. METHODS: Clinically stable bronchiectasispatients were studied prospectively at two centres. Patients with other pulmonary conditions were excluded. Spontaneous sputum was subject to bacterial culture, and secreted mucins (MUC2, MUC5AC and MUC5B) were measured in sputum supernatants by ELISA. RESULTS: A total of 50 patients were included. PPM were identified from sputum samples in 30 (60%), with Pseudomonas aeruginosa (n = 10) and Haemophilus influenzae (n = 10) as the most common PPM. There were no baseline differences among airway colonized and non-colonized patients. Patients with airways colonized by PPM presented higher levels of airway MUC2. No differences in MUC5AC levels were found among groups, whereas MUC5B levels were undetectable. Patients with P. aeruginosa colonization expressed the highest levels of MUC2. High levels of MUC2 and MUC5AC are also correlated with disease severity using the Bronchiectasis Severity Index. CONCLUSIONS: Airway MUC2 levels were higher in bronchiectasispatients colonized with PPM compared with those without airway colonization, especially in patients with P. aeruginosa. These findings suggest that airway-secreted mucins levels may play a role in the pathogenesis of airway infection in non-CF bronchiectasis.
Authors: Kathryn A Ramsey; Alice C H Chen; Giorgia Radicioni; Rohan Lourie; Megan Martin; Amy Broomfield; Yong H Sheng; Sumaira Z Hasnain; Graham Radford-Smith; Lisa A Simms; Lucy Burr; David J Thornton; Simon D Bowler; Stephanie Livengood; Agathe Ceppe; Michael R Knowles; Peadar G Noone; Scott H Donaldson; David B Hill; Camille Ehre; Brian Button; Neil E Alexis; Mehmet Kesimer; Richard C Boucher; Michael A McGuckin Journal: Am J Respir Crit Care Med Date: 2020-03-15 Impact factor: 21.405
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