Ulrik Lausten-Thomsen1, Michael Christiansen2, Cilius Esmann Fonvig3, Cæcilie Trier3, Oluf Pedersen4, Torben Hansen5, Jens-Christian Holm6. 1. The Children's Obesity Clinic, Department of Pediatrics, Copenhagen University Hospital Holbæk, Holbæk, Denmark; Department of Clinical Medicine, Copenhagen University, Copenhagen, Denmark. Electronic address: ult@dadlnet.dk. 2. Department of Clinical Biochemistry, Immunology and Genetics, Statens Serum Institut, Copenhagen, Denmark. 3. The Children's Obesity Clinic, Department of Pediatrics, Copenhagen University Hospital Holbæk, Holbæk, Denmark. 4. The Novo Nordisk Foundation Center for Basic Metabolic Research, Copenhagen, Denmark. 5. The Novo Nordisk Foundation Center for Basic Metabolic Research, Copenhagen, Denmark; Faculty of Health Sciences, University of Southern Denmark, Odense, Denmark. 6. The Children's Obesity Clinic, Department of Pediatrics, Copenhagen University Hospital Holbæk, Holbæk, Denmark; Department of Clinical Medicine, Copenhagen University, Copenhagen, Denmark.
Abstract
BACKGROUND: Adiponectin is an abundant adipocyte-secreted hormone that modulates a number of metabolic processes and is correlated to various metabolic disorders. Pediatric reference levels are needed for the risk stratification and interpretation of individual serum adiponectin levels. METHODS: A total of 1193 healthy, non-obese Danish schoolchildren (730 girls, 463 boys) aged 6-18 years (median 11.9) were examined by trained medical staff. Total serum adiponectin concentrations in venous fasting blood samples were quantitated by a DuoSet® ELISA human Adiponectin/Acrp30 (R&D Systems) following optimization. RESULTS: In a generalized linear model adjusted for BMI SDS, total serum adiponectin concentrations were correlated to age in girls (p<0.0001) and boys (p=<0.0001) and for both sexes combined (p<0.0001). No significant difference between sexes was found. Reference intervals were calculated using age as a continuous variable. The best fitted reference curve for both sexes was: 50th percentile: Y=-0.1478 ∗ X+6.046; 2.5th percentile: Y=-0.06256 ∗ X+2.34; 97.5th percentile: Y=-0.4086 ∗ X+22.39, where Y=adiponectin in μg/mL and X=years of age (from 6 to 18 years). CONCLUSION: We developed a pediatric reference levels for total serum adiponectin in a sample of 1193 Danish children and adolescents aged 6-18 years. A correlation with age was demonstrated in children, but no significant difference was seen between the sexes.
BACKGROUND:Adiponectin is an abundant adipocyte-secreted hormone that modulates a number of metabolic processes and is correlated to various metabolic disorders. Pediatric reference levels are needed for the risk stratification and interpretation of individual serum adiponectin levels. METHODS: A total of 1193 healthy, non-obese Danish schoolchildren (730 girls, 463 boys) aged 6-18 years (median 11.9) were examined by trained medical staff. Total serum adiponectin concentrations in venous fasting blood samples were quantitated by a DuoSet® ELISA humanAdiponectin/Acrp30 (R&D Systems) following optimization. RESULTS: In a generalized linear model adjusted for BMI SDS, total serum adiponectin concentrations were correlated to age in girls (p<0.0001) and boys (p=<0.0001) and for both sexes combined (p<0.0001). No significant difference between sexes was found. Reference intervals were calculated using age as a continuous variable. The best fitted reference curve for both sexes was: 50th percentile: Y=-0.1478 ∗ X+6.046; 2.5th percentile: Y=-0.06256 ∗ X+2.34; 97.5th percentile: Y=-0.4086 ∗ X+22.39, where Y=adiponectin in μg/mL and X=years of age (from 6 to 18 years). CONCLUSION: We developed a pediatric reference levels for total serum adiponectin in a sample of 1193 Danish children and adolescents aged 6-18 years. A correlation with age was demonstrated in children, but no significant difference was seen between the sexes.
Authors: Alberto Romano; Ester Del Vescovo; Serena Rivetti; Silvia Triarico; Giorgio Attinà; Stefano Mastrangelo; Palma Maurizi; Antonio Ruggiero Journal: J Pers Med Date: 2022-05-27
Authors: Sara E Stinson; Anna E Jonsson; Morten A V Lund; Christine Frithioff-Bøjsøe; Louise Aas Holm; Oluf Pedersen; Lars Ängquist; Thorkild I A Sørensen; Jens J Holst; Michael Christiansen; Jens-Christian Holm; Bolette Hartmann; Torben Hansen Journal: J Clin Endocrinol Metab Date: 2021-05-13 Impact factor: 5.958
Authors: Sara E Stinson; Anna E Jonsson; Ierai Fernández de Retana Alzola; Morten A V Lund; Christine Frithioff-Bøjsøe; Louise Aas Holm; Cilius E Fonvig; Oluf Pedersen; Lars Ängquist; Thorkild I A Sørensen; Jens J Holst; Michael Christiansen; Jens-Christian Holm; Bolette Hartmann; Torben Hansen Journal: J Clin Endocrinol Metab Date: 2022-05-17 Impact factor: 6.134