| Literature DB >> 26168732 |
Hana Nůsková1, Tomáš Mráček2, Tereza Mikulová2, Marek Vrbacký2, Nikola Kovářová2, Jana Kovalčíková2, Petr Pecina2, Josef Houštěk3.
Abstract
Mitochondrial ATP synthase, ADP/ATP translocase (ANT), and inorganic phosphate carrier (PiC) are supposed to form a supercomplex called ATP synthasome. Our protein and transcript analysis of rat tissues indicates that the expression of ANT and PiC is transcriptionally controlled in accordance with the biogenesis of ATP synthase. In contrast, the content of ANT and PiC is increased in ATP synthase deficient patients' fibroblasts, likely due to a post-transcriptional adaptive mechanism. A structural analysis of rat heart mitochondria by immunoprecipitation, blue native/SDS electrophoresis, immunodetection and MS analysis revealed the presence of ATP synthasome. However, the majority of PiC and especially ANT did not associate with ATP synthase, suggesting that most of PiC, ANT and ATP synthase exist as separate entities.Entities:
Keywords: ATP synthasome; Mitochondria; Oxidative phosphorylation; Supercomplexes
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Year: 2015 PMID： 26168732 DOI： 10.1016/j.bbrc.2015.07.034
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575