Akane Yonehara1, Yuka Tanaka2, Kasem Kulkeaw3, Takumi Era4, Yoichi Nakanishi1, Daisuke Sugiyama5. 1. Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, Japan. 2. Center for Advanced Medical Innovation, Kyushu University, Fukuoka, Japan Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan. 3. Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan. 4. Department of Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan. 5. Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, Japan Center for Advanced Medical Innovation, Kyushu University, Fukuoka, Japan ds-mons@yb3.so-net.ne.jp.
Abstract
BACKGROUND/AIM: Neuroblastoma is a pediatric solid tumor refractory to eradication by chemotherapy. To determine whether Aloe vera (AV), a pontial anticancer reagent, could be useful in neuroblastoma therapy, we investigated the anti-proliferative effects of an AV protein extract. MATERIALS AND METHODS: Human neuroblastoma cell lines (IMR-32, TGW, CHP-126 and NBL-S) were cultured with AV protein extract and proliferation status was assessed by cell counting, Ki-67 staining and gene expression. RESULTS: Among tested lines, the number of viable, AV-treated IMR-32 cells significantly decreased 1.98-fold by day 2 and 1.33-fold by day 5 of culture relative to untreated controls (p<0.05). Treatment also decreased the number of Ki-67(+) IMR-32 cells by 13% by day 5 (p<0.05) and, unlike untreated controls, CCND2 mRNA expression levels became undetectable by day 1. CONCLUSION: AV-protein extract suppresses human IMR-32 neuroblastoma cell proliferation, possibly by suppressing CCND2 transcript levels in vitro. Copyright
BACKGROUND/AIM: Neuroblastoma is a pediatric solid tumor refractory to eradication by chemotherapy. To determine whether Aloe vera (AV), a pontial anticancer reagent, could be useful in neuroblastoma therapy, we investigated the anti-proliferative effects of an AV protein extract. MATERIALS AND METHODS:Humanneuroblastoma cell lines (IMR-32, TGW, CHP-126 and NBL-S) were cultured with AV protein extract and proliferation status was assessed by cell counting, Ki-67 staining and gene expression. RESULTS: Among tested lines, the number of viable, AV-treated IMR-32 cells significantly decreased 1.98-fold by day 2 and 1.33-fold by day 5 of culture relative to untreated controls (p<0.05). Treatment also decreased the number of Ki-67(+) IMR-32 cells by 13% by day 5 (p<0.05) and, unlike untreated controls, CCND2 mRNA expression levels became undetectable by day 1. CONCLUSION:AV-protein extract suppresses human IMR-32 neuroblastoma cell proliferation, possibly by suppressing CCND2 transcript levels in vitro. Copyright