Yu Sumiya1, Mami Ishikawa1, Takahiro Inoue1, Toshio Inui2, Daisuke Kuchiike3, Kentaro Kubo4, Yoshihiro Uto5, Takahito Nishikata6. 1. Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan. 2. Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan Saisei Mirai Cell Processing Center, Osaka, Japan Inui Immunotherapy Clinic, Osaka, Japan. 3. Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan Saisei Mirai Cell Processing Center, Osaka, Japan. 4. Saisei Mirai Cell Processing Center, Osaka, Japan. 5. Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan. 6. Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan nisikata@konan-u.ac.jp.
Abstract
BACKGROUND: Although the mechanisms of macrophage activation are important for cancer immunotherapy, they are poorly understood. Recently, easy and robust assay systems for assessing the macrophage-activating factor (MAF) using monocytic cell line-derived macrophages were established. MATERIALS AND METHODS: Gene-expression profiles of U937- and THP-1-derived macrophages were compared using gene expression microarray analysis and their responses against several MAFs were examined by in vitro experiments. RESULTS: Activated states of these macrophages could not be assigned to a specific sub-type but showed, however, different unique characteristics. CONCLUSION: The unique of monocytic cell line-derived macrophages could provide clues to understand the activation mechanism of macrophages and, therefore, help to develop effective cancer immunotherapy with MAFs. Copyright
BACKGROUND: Although the mechanisms of macrophage activation are important for cancer immunotherapy, they are poorly understood. Recently, easy and robust assay systems for assessing the macrophage-activating factor (MAF) using monocytic cell line-derived macrophages were established. MATERIALS AND METHODS: Gene-expression profiles of U937- and THP-1-derived macrophages were compared using gene expression microarray analysis and their responses against several MAFs were examined by in vitro experiments. RESULTS: Activated states of these macrophages could not be assigned to a specific sub-type but showed, however, different unique characteristics. CONCLUSION: The unique of monocytic cell line-derived macrophages could provide clues to understand the activation mechanism of macrophages and, therefore, help to develop effective cancer immunotherapy with MAFs. Copyright
Authors: Serena Tedesco; Federica De Majo; Jieun Kim; Annalisa Trenti; Lucia Trevisi; Gian Paolo Fadini; Chiara Bolego; Peter W Zandstra; Andrea Cignarella; Libero Vitiello Journal: Front Pharmacol Date: 2018-02-22 Impact factor: 5.810