Takahiro Inoue1, Mami Ishikawa1, Yu Sumiya1, Haruka Kohda1, Toshio Inui2, Daisuke Kuchiike3, Kentaro Kubo4, Yoshihiro Uto5, Takahito Nishikata6. 1. Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan. 2. Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan Saisei Mirai Cell Processing Center, Osaka, Japan Inui Immunotherapy Clinic, Osaka, Japan. 3. Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan Saisei Mirai Cell Processing Center, Osaka, Japan. 4. Saisei Mirai Cell Processing Center, Osaka, Japan. 5. Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan. 6. Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan nisikata@konan-u.ac.jp.
Abstract
BACKGROUND: As the mechanism of macrophage activation is not well-understood, standardization of an assay system for measuring phagocytic activities of macrophages will be useful for research on macrophages. Previously, we established a novel standardized macrophage-activating factor (MAF) assay system using U937. MATERIALS AND METHODS: Using the human monocytic cell line THP-1, another standardized MAF assay system was established. Characteristic gene expression of U937- and THP-1-derived macrophages was compared by gene expression microarray analysis. RESULTS: Both U937- and THP-1-derived macrophages showed obvious phagocytic activities with unique characteristics and, therefore, could not be assigned to a single sub-type. CONCLUSION: Activation of macrophages is an intricate cellular process. Comparison of our two novel assay systems provides new insights into macrophage activation mechanisms. Copyright
BACKGROUND: As the mechanism of macrophage activation is not well-understood, standardization of an assay system for measuring phagocytic activities of macrophages will be useful for research on macrophages. Previously, we established a novel standardized macrophage-activating factor (MAF) assay system using U937. MATERIALS AND METHODS: Using the human monocytic cell line THP-1, another standardized MAF assay system was established. Characteristic gene expression of U937- and THP-1-derived macrophages was compared by gene expression microarray analysis. RESULTS: Both U937- and THP-1-derived macrophages showed obvious phagocytic activities with unique characteristics and, therefore, could not be assigned to a single sub-type. CONCLUSION: Activation of macrophages is an intricate cellular process. Comparison of our two novel assay systems provides new insights into macrophage activation mechanisms. Copyright