Suleyman Turedi1, Ozgur Tatli2, Ahmet Alver3, Ersagun Karaguzel4, Yunus Karaca5, Suha Turkmen5, Esin Yulug6, Aysegul Sumer3, Diler Us Altay3, Ahmet Mentese3. 1. Department of Emergency Medicine, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey. Electronic address: suleymanturedi@hotmail.com. 2. Department of Emergency Medicine, Kanuni Training and Research Hospital, Trabzon, Turkey. 3. Department of Clinical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey. 4. Department of Clinical Urology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey. 5. Department of Emergency Medicine, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey. 6. Department of Histology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
Abstract
OBJECTIVE: To investigate the potential diagnostic value of plasma signal peptide, CUB (complement proteins C1r/C1s, Uegf, Bmp1) domain, epidermal growth factor (EGF)-like 1 (SCUBE1) protein in experimentally induced testicular torsion (TT). MATERIALS AND METHODS: In this randomized, controlled, experimental study, 24 mature male Wistar rats were divided into four groups: 2- and 4-hour control (groups I and III, respectively), and 2- and 4-hour torsion (groups II and IV, respectively) groups. Torsion was performed by rotating the left testis 720° clockwise and maintained by fixing the testis. Plasma SCUBE1 levels and histopathological damage scores were compared. RESULTS: There was significantly greater histopathological damage in the 4-hour torsion group compared with the other groups. SCUBE1 levels in this group were also higher than those in the other groups, and the difference was significant. There were significant correlations between histopathological scores and SCUBE1 levels. CONCLUSION: SCUBE1, a novel marker of platelet activation, is elevated in TT. According to our results, platelet activation may play an important pathological role in tissue injury associated with testicular ischemia. Plasma SCUBE1 measurement may have diagnostic, therapeutic, or prognostic value in TT.
OBJECTIVE: To investigate the potential diagnostic value of plasma signal peptide, CUB (complement proteins C1r/C1s, Uegf, Bmp1) domain, epidermal growth factor (EGF)-like 1 (SCUBE1) protein in experimentally induced testicular torsion (TT). MATERIALS AND METHODS: In this randomized, controlled, experimental study, 24 mature male Wistar rats were divided into four groups: 2- and 4-hour control (groups I and III, respectively), and 2- and 4-hour torsion (groups II and IV, respectively) groups. Torsion was performed by rotating the left testis 720° clockwise and maintained by fixing the testis. Plasma SCUBE1 levels and histopathological damage scores were compared. RESULTS: There was significantly greater histopathological damage in the 4-hour torsion group compared with the other groups. SCUBE1 levels in this group were also higher than those in the other groups, and the difference was significant. There were significant correlations between histopathological scores and SCUBE1 levels. CONCLUSION:SCUBE1, a novel marker of platelet activation, is elevated in TT. According to our results, platelet activation may play an important pathological role in tissue injury associated with testicular ischemia. Plasma SCUBE1 measurement may have diagnostic, therapeutic, or prognostic value in TT.
Authors: Ignacio Del Valle; Federica Buonocore; Andrew J Duncan; Lin Lin; Martino Barenco; Rahul Parnaik; Sonia Shah; Mike Hubank; Dianne Gerrelli; John C Achermann Journal: Wellcome Open Res Date: 2017-04-07