| Literature DB >> 26166432 |
Farhat Ullah1, Muhammad Ayaz1, Abdul Sadiq1, Abid Hussain1, Sajjad Ahmad1, Muhammad Imran1, Anwar Zeb1.
Abstract
This study was designed to investigate antioxidant and anticholinesterase potential of Iris germanica var; florentina. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory potential of plant samples were investigated by Ellman's assay. Antioxidant activity was performed using DPPH, H2O2 and ABTS free radical scavenging assays. Total phenolics and flavonoids contents were expressed in mg GAE/g dry weight and mg RTE/g, respectively. In AChE inhibition assay, Ig.Fl, Ig.Sp and Ig.Cf fractions exhibited highest activity with IC50 values of < 0.1, 5.64 and 19 μg/mL, respectively. In BChE inhibitory assay, Ig.Fl, Ig.Sp, Ig.Cf and Ig.Cr were most active with IC50 of < 0.1, < 0.1, 31 and 78 μg/mL, respectively. In DPPH assay, Ig.Fl and Ig.Cf exhibited highest inhibition of free radicals, 80.52% (IC50 = 9 μg/mL) and 78.30% (IC50 = 8 μg/mL), respectively. In ABTS assay Ig.Cr, Ig.Cf, Ig.Fl and Ig.Sp exhibited IC50 values of < 0.1, 2, 2 and 3 μg/mL, respectively.Entities:
Keywords: ABTS; Acetylcholinesterase (AChE); DPPH; Ellman’s assay; H2O2; Iris germanica; butyrylcholinesterase (BChE)
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Year: 2015 PMID： 26166432 DOI： 10.1080/14786419.2015.1057585
Source DB: PubMed Journal: Nat Prod Res ISSN： 1478-6419 Impact factor: 2.861