Traian Popescu1, Andreea R Lupu2, Valentin Raditoiu3, Violeta Purcar3, Valentin S Teodorescu4. 1. National Institute of Materials Physics, P.O. Box MG-7, 077125 Bucharest, Romania; University of Bucharest, Faculty of Physics, 077125 Bucharest, Romania. Electronic address: tr.popescu@gmail.com. 2. Cantacuzino National Institute for Research and Development in Microbiology and Immunology, 050096 Bucharest, Romania; Victor Babes National Institute of Pathology, 050096 Bucharest, Romania. 3. National Research and Development Institute for Chemistry and Petrochemistry-ICECHIM, 060021 Bucharest, Romania. 4. National Institute of Materials Physics, P.O. Box MG-7, 077125 Bucharest, Romania.
Abstract
HYPOTHESIS: The MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide] cell cytotoxicity indicator is photocatalytically reduced on the surface of TiO2 nanoparticles in phosphate-buffered-saline (PBS) environment. We hypothesize that specific phosphate adsorption may be used to modulate the efficiency of the TiO2-MTT reaction through colloidal and semiconductor-liquid interface processes. EXPERIMENTS: The TiO2-MTT reaction kinetics was studied in PBS, with respect to photocatalyst and MTT concentrations and irradiation wavelength. The effects of PBS and electron scavengers (Fe(3+) ions) on reaction efficiency and the role of colloidal surface charge in the photocatalytic process were investigated. The structural and spectroscopic characteristics of relevant TiO2-formazan systems were studied by X-ray diffraction, transmission electron microscopy and IR-spectroscopy. FINDINGS: The reaction was pseudo-first order with respect to photocatalyst and showed a negative and fractional partial order with respect to MTT. Formazan production rates were directly proportional to radiation wavelength and TiO2 concentration and inversely proportional to the MTT initial concentration. The addition of Fe(3+) ions, as well as the absence of PBS, induced strong reaction inhibition. Reaction efficiency and catalyst Zeta potential were enhanced by Na2HPO4 (PBS component) and showed a maximum around the phosphate concentration 0.005 M. Structural/spectroscopic characterization confirmed the formation of amorphous MTT-formazan on the surface of TiO2 and the TiO2-phosphate binding.
HYPOTHESIS: The MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide] cell cytotoxicity indicator is photocatalytically reduced on the surface of TiO2 nanoparticles in phosphate-buffered-saline (PBS) environment. We hypothesize that specific phosphate adsorption may be used to modulate the efficiency of the TiO2-MTT reaction through colloidal and semiconductor-liquid interface processes. EXPERIMENTS: The TiO2-MTT reaction kinetics was studied in PBS, with respect to photocatalyst and MTT concentrations and irradiation wavelength. The effects of PBS and electron scavengers (Fe(3+) ions) on reaction efficiency and the role of colloidal surface charge in the photocatalytic process were investigated. The structural and spectroscopic characteristics of relevant TiO2-formazan systems were studied by X-ray diffraction, transmission electron microscopy and IR-spectroscopy. FINDINGS: The reaction was pseudo-first order with respect to photocatalyst and showed a negative and fractional partial order with respect to MTT. Formazan production rates were directly proportional to radiation wavelength and TiO2 concentration and inversely proportional to the MTT initial concentration. The addition of Fe(3+) ions, as well as the absence of PBS, induced strong reaction inhibition. Reaction efficiency and catalyst Zeta potential were enhanced by Na2HPO4 (PBS component) and showed a maximum around the phosphate concentration 0.005 M. Structural/spectroscopic characterization confirmed the formation of amorphous MTT-formazan on the surface of TiO2 and the TiO2-phosphate binding.
Authors: Kashif Maqbool Khan; Lutfun Nahar; Abdul Mannan; Muhammad Arfan; Ghazanfar Ali Khan; Afaf Al-Groshi; Andrew Evans; Nicola M Dempster; Fyaz M D Ismail; Satyajit D Sarker Journal: Pharmacogn Mag Date: 2018-01-31 Impact factor: 1.085