Literature DB >> 26160573

In Situ Proximity Ligation Assay (PLA).

Sonchita Bagchi1, Robert Fredriksson, Åsa Wallén-Mackenzie.   

Abstract

In situ proximity ligation assay (PLA) is a method to identify physical closeness of proteins, where a signal will only be produced if the two proteins are closer than 40 nm, in tissue section or cell cultures. Modifications of the PLA method can also be used to increase specificity or sensitivity of standard immunohistochemistry protocols.

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Year:  2015        PMID： 26160573     DOI： 10.1007/978-1-4939-2742-5_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  48 in total

1.  Assessing In Situ Phosphoinositide-Protein Interactions Through Fluorescence Proximity Ligation Assay in Cultured Cells.

Authors:  Mo Chen; Hudson T Horn; Tianmu Wen; Vincent L Cryns; Richard A Anderson
Journal:  Methods Mol Biol       Date:  2021

2.  Arrestin-β-1 Physically Scaffolds TSH and IGF1 Receptors to Enable Crosstalk.

Authors:  Christine C Krieger; Alisa Boutin; Daesong Jang; Sarah J Morgan; J Paul Banga; George J Kahaly; Joanna Klubo-Gwiezdzinska; Susanne Neumann; Marvin C Gershengorn
Journal:  Endocrinology       Date:  2019-06-01       Impact factor: 4.736

3.  Protein phosphatase 2A-mediated flotillin-1 dephosphorylation up-regulates endothelial cell migration and angiogenesis regulation.

Authors:  Zsófia Thalwieser; Nikolett Király; Márton Fonódi; Csilla Csortos; Anita Boratkó
Journal:  J Biol Chem       Date:  2019-11-21       Impact factor: 5.157

4.  NHERF1 Is Required for Localization of PMCA2 and Suppression of Early Involution in the Female Lactating Mammary Gland.

Authors:  Jaekwang Jeong; Wonnam Kim; Julie Hens; Pamela Dann; Pepper Schedin; Peter A Friedman; John J Wysolmerski
Journal:  Endocrinology       Date:  2019-08-01       Impact factor: 4.736

5.  Calcineurin Aβ-Specific Anchoring Confers Isoform-Specific Compartmentation and Function in Pathological Cardiac Myocyte Hypertrophy.

Authors:  Xiaofeng Li; Jinliang Li; Eliana C Martinez; Alexander Froese; Catherine L Passariello; Kathryn Henshaw; Francesca Rusconi; Yang Li; Qian Yu; Hrishikesh Thakur; Viacheslav O Nikolaev; Michael S Kapiloff
Journal:  Circulation       Date:  2020-07-02       Impact factor: 29.690

6.  Tetraspanin CD82 Organizes Dectin-1 into Signaling Domains to Mediate Cellular Responses to Candida albicans.

Authors:  Jenny M Tam; Jennifer L Reedy; Daniel P Lukason; Sunnie G Kuna; Mridu Acharya; Nida S Khan; Paige E Negoro; Shuying Xu; Rebecca A Ward; Michael B Feldman; Richard A Dutko; Jane B Jeffery; Anna Sokolovska; Carl N Wivagg; Kara G Lassen; François Le Naour; Vasiliki Matzaraki; Ethan C Garner; Ramnik J Xavier; Vinod Kumar; Frank L van de Veerdonk; Mihai G Netea; Cindy K Miranti; Michael K Mansour; Jatin M Vyas
Journal:  J Immunol       Date:  2019-04-22       Impact factor: 5.422

7.  Endogenous Serotonin 5-HT2A and 5-HT2C Receptors Associate in the Medial Prefrontal Cortex.

Authors:  Amanda E Price; Dennis J Sholler; Sonja J Stutz; Noelle C Anastasio; Kathryn A Cunningham
Journal:  ACS Chem Neurosci       Date:  2019-03-18       Impact factor: 4.418

8.  An intranasally administered monoclonal antibody cocktail abrogates ricin toxin-induced pulmonary tissue damage and inflammation.

Authors:  Yinghui Rong; Fernando J Torres-Velez; Dylan Ehrbar; Jennifer Doering; Renjie Song; Nicholas J Mantis
Journal:  Hum Vaccin Immunother       Date:  2019-10-29       Impact factor: 3.452

9.  Mitochondria-Endoplasmic Reticulum Contact Sites Function as Immunometabolic Hubs that Orchestrate the Rapid Recall Response of Memory CD8+ T Cells.

Authors:  Glenn R Bantug; Marco Fischer; Jasmin Grählert; Maria L Balmer; Gunhild Unterstab; Leyla Develioglu; Rebekah Steiner; Lianjun Zhang; Ana S H Costa; Patrick M Gubser; Anne-Valérie Burgener; Ursula Sauder; Jordan Löliger; Réka Belle; Sarah Dimeloe; Jonas Lötscher; Annaïse Jauch; Mike Recher; Gideon Hönger; Michael N Hall; Pedro Romero; Christian Frezza; Christoph Hess
Journal:  Immunity       Date:  2018-03-06       Impact factor: 31.745

10.  Proximity Ligation Assay for Detecting Protein-Protein Interactions and Protein Modifications in Cells and Tissues in Situ.

Authors:  Marihan Hegazy; Eran Cohen-Barak; Jennifer L Koetsier; Nicole A Najor; Constadina Arvanitis; Eli Sprecher; Kathleen J Green; Lisa M Godsel
Journal:  Curr Protoc Cell Biol       Date:  2020-12
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