Maria Messina1, Claudia Ariaudo1, Loredana Praticò Barbato2, Silvia Beltramo3, Gianna Mazzucco4, Antonio Amoroso2, Andrea Ranghino1, Vincenzo Cantaluppi5, Fabrizio Fop1, Giuseppe Paolo Segoloni1, Luigi Biancone6. 1. Renal Transplantation Unit 'A. Vercellone', Division of Nephrology Dialysis and Transplantation, AOU Città della Salute e della Scienza di Torino and Department of Medical Sciences, University of Turin, Italy. 2. Immunogenetics and Transplant Biology Service, AOU Città della Salute e della Scienza di Torino and Department of Medical Sciences, University of Turin, Italy. 3. Center for Experimental Medical Research (CeRMS), University of Turin, Italy. 4. Division of Pathology Transplantation, Department of Medical Sciences, University of Turin, Italy. 5. Renal Transplantation Unit 'A. Vercellone', Division of Nephrology Dialysis and Transplantation, AOU Città della Salute e della Scienza di Torino and Department of Medical Sciences, University of Turin, Italy; Center for Experimental Medical Research (CeRMS), University of Turin, Italy. 6. Renal Transplantation Unit 'A. Vercellone', Division of Nephrology Dialysis and Transplantation, AOU Città della Salute e della Scienza di Torino and Department of Medical Sciences, University of Turin, Italy; Center for Experimental Medical Research (CeRMS), University of Turin, Italy. Electronic address: luigi.biancone@unito.it.
Abstract
BACKGROUND: The C1q-binding properties of donor specific antibodies (DSA) may be related to antibody-mediated rejection and poor outcome. METHODS: We retrospectively studied 35 kidney transplant recipients with transplant glomerulopathy (TG) and de novo DSA (dnDSA). C1q dnDSA were measured in the serum stored at renal biopsy and the association among C1q-fixing dnDSA, C4d deposition and graft loss was examined. RESULTS: Of the 35 patients with dnDSA and TG, 15 (42.9%) had C1q-positive dnDSA and 20 (57.1%) had C1q-negative dnDSA. Ten out of 15 patients with C1q-positive dnDSA (66.6%) and 5 with C1q-negative dnDSA (25%) had C4d positive staining renal biopsies (P=0.02), being the C1q-negative dnDSA/C4d-negative TG 42.9% of the total. The C1q-positive dnDSA group has significantly higher IgG DSA Class II MFI than the C1q-negative dnDSA group (P=0.004). Patients with C4d deposits have significantly higher IgG DSA MFI for both Class I and Class II than those without C4d deposits (P=0.02). We found a trend toward higher graft loss in the C1q-positive dnDSA group (60%) versus the C1q-negative dnDSA group (40%) without a statistical significance (P=0.31). CONCLUSION: Our study provides further characterization of TG associated with dnDSA. The major part of dnDSA-associated TG was C1q-negative and the presence of C1q-fixing dnDSA did not significantly correlate with graft outcome.
BACKGROUND: The C1q-binding properties of donor specific antibodies (DSA) may be related to antibody-mediated rejection and poor outcome. METHODS: We retrospectively studied 35 kidney transplant recipients with transplant glomerulopathy (TG) and de novo DSA (dnDSA). C1qdnDSA were measured in the serum stored at renal biopsy and the association among C1q-fixing dnDSA, C4d deposition and graft loss was examined. RESULTS: Of the 35 patients with dnDSA and TG, 15 (42.9%) had C1q-positive dnDSA and 20 (57.1%) had C1q-negative dnDSA. Ten out of 15 patients with C1q-positive dnDSA (66.6%) and 5 with C1q-negative dnDSA (25%) had C4d positive staining renal biopsies (P=0.02), being the C1q-negative dnDSA/C4d-negative TG 42.9% of the total. The C1q-positive dnDSA group has significantly higher IgG DSA Class II MFI than the C1q-negative dnDSA group (P=0.004). Patients with C4d deposits have significantly higher IgG DSA MFI for both Class I and Class II than those without C4d deposits (P=0.02). We found a trend toward higher graft loss in the C1q-positive dnDSA group (60%) versus the C1q-negative dnDSA group (40%) without a statistical significance (P=0.31). CONCLUSION: Our study provides further characterization of TG associated with dnDSA. The major part of dnDSA-associated TG was C1q-negative and the presence of C1q-fixing dnDSA did not significantly correlate with graft outcome.
Authors: Rachel M Engen; Giulia E Park; Cooper S Schumacher; Idoia Gimferrer; Paul Warner; Laura S Finn; Noel S Weiss; Jodi M Smith Journal: Transplantation Date: 2018-12 Impact factor: 4.939
Authors: Alberto Mella; Ester Gallo; Maria Messina; Cristiana Caorsi; Antonio Amoroso; Paolo Gontero; Aldo Verri; Francesca Maletta; Antonella Barreca; Fabrizio Fop; Luigi Biancone Journal: World J Transplant Date: 2018-09-10