Massimo Re1, Artan Çeka2, Corrado Rubini3, Luigi Ferrante4, Antonio Zizzi3, Federico M Gioacchini1, Michele Tulli1, Liana Spazzafumo5, Stefano Sellari-Franceschini6, Antonio D Procopio2,7, Fabiola Olivieri2,7. 1. Department of Otorhinolaryngology, Umberto I University General Hospital, Polytechnic University of the Marches, Ancona, Italy. 2. Department of Molecular and Clinical Sciences, Polytechnic University of the Marches, Ancona, Italy. 3. Pathologic Anatomy and Histopathology Division, Department of Biomedical Sciences and Public Health, Polytechnic University of the Marches, Ancona, Italy. 4. Department of Biomedical Sciences and Public Health, Section of Medical Statistics, Faculty of Medicine, Polytechnic University of the Marches, Ancona, Italy. 5. Center of Biostatistics, National Institute of Repose and Cure for Elderly-Scientific Institute for Hospitalization and Treatment (INRCA-IRCCS) Italian National Institute, Ancona, Italy. 6. Department of Otorhinolaryngology, University of Pisa, Pisa, Italy. 7. Center of Clinical Pathology and Innovative Therapy, INRCA-IRCCS Italian National Institute, Ancona, Italy.
Abstract
OBJECTIVES/HYPOTHESIS: Altered microRNA expression has been found in many cancer types, including laryngeal squamous cell carcinoma (LSCC). We investigated the association of LSCC-related miR-34c-5p with disease-free survival and overall survival. STUDY DESIGN: Retrospective cohort study. METHODS: Expression levels of miR-34c-5p were detected in 90 LSCC formalin-fixed paraffin-embedded tissues by reverse-transcription quantitative polymerase chain reaction. Overall survival and disease-free survival were evaluated using the Kaplan-Meier method, and multivariate analysis was performed using Cox proportional hazard analysis. RESULTS: A downregulation of miR-34c-5p expression significantly correlated with worse disease-free and overall survival. In the multivariate analysis, low miR-34c-5p expression was associated with an increased risk of recurrence. CONCLUSIONS: A downregulation of miR-34c-5p in LSCC is independently associated with unfavorable disease-free survival, suggesting that miR-34c-5p might be a promising marker for evaluating the risk of recurrences. LEVEL OF EVIDENCE: NA.
OBJECTIVES/HYPOTHESIS: Altered microRNA expression has been found in many cancer types, including laryngeal squamous cell carcinoma (LSCC). We investigated the association of LSCC-related miR-34c-5p with disease-free survival and overall survival. STUDY DESIGN: Retrospective cohort study. METHODS: Expression levels of miR-34c-5p were detected in 90 LSCC formalin-fixed paraffin-embedded tissues by reverse-transcription quantitative polymerase chain reaction. Overall survival and disease-free survival were evaluated using the Kaplan-Meier method, and multivariate analysis was performed using Cox proportional hazard analysis. RESULTS: A downregulation of miR-34c-5p expression significantly correlated with worse disease-free and overall survival. In the multivariate analysis, low miR-34c-5p expression was associated with an increased risk of recurrence. CONCLUSIONS: A downregulation of miR-34c-5p in LSCC is independently associated with unfavorable disease-free survival, suggesting that miR-34c-5p might be a promising marker for evaluating the risk of recurrences. LEVEL OF EVIDENCE: NA.
Authors: Massimo Re; Giuseppe Magliulo; Federico M Gioacchini; Arisa Bajraktari; Andrea Bertini; Artan Çeka; Corrado Rubini; Luigi Ferrante; Antonio D Procopio; Fabiola Olivieri Journal: Biomed Res Int Date: 2017-08-31 Impact factor: 3.411
Authors: Joshua Lubov; Mariana Maschietto; Iman Ibrahim; Alex Mlynarek; Michael Hier; Luiz Paulo Kowalski; Moulay A Alaoui-Jamali; Sabrina Daniela da Silva Journal: Oncotarget Date: 2017-07-13
Authors: Zuzana Vojtechova; Ivan Sabol; Martina Salakova; Jana Smahelova; Jiri Zavadil; Lubomir Turek; Marek Grega; Jan Klozar; Bohumir Prochazka; Ruth Tachezy Journal: BMC Cancer Date: 2016-07-04 Impact factor: 4.430