Xiuqing Ma1, Junchang Cui1, Jing Wang2, Yan Chang3, Qiuhong Fang2, Changqing Bai4, Xiumei Zhou5, Hong Zhou6, Huasong Feng7, Ying Wang3, Weiguo Zhao8, Zhongguang Wen9, Ping Wang10, Yi Liu11, Ling Yu1, Chunsun Li1, Liangan Chen12. 1. Department of Respiratory Medicine, Chinese People's Liberation Army General Hospital, Beijing, China. 2. Department of Pulmonary and Critical Care Medicine, Beijing Shijitan Hospital, Beijing, China. 3. Department of Respiratory Medicine, the PLA Second Artillery Force General Hospital, Beijing, China. 4. Department of Respiratory Medicine, PLA 307 Hospital, Beijing, China. 5. Department of Respiratory Medicine, Beijing Fengtai Hospital, Beijing, China. 6. Department of Respiratory Medicine, Beijing Electric Power Hospital, Beijing, China. 7. Department of Respiratory Medicine, Navy General Hospital, Beijing, China. 8. Department of Respiratory Medicine, PLA 309 Hospital, Beijing, China. 9. Department of Respiratory Medicine, PLA 304 Hospital, Beijing, China. 10. Department of Respiratory Medicine, PLA 306 Hospital, Beijing, China. 11. Department of Respiratory Medicine, Airforce General Hospital, Beijing, China. 12. Department of Respiratory Medicine, Chinese People's Liberation Army General Hospital, Beijing, China chenliangan301@163.com.
Abstract
OBJECTIVE: A prospective observational study to investigate the distribution and antimicrobial resistance of pathogenic bacteria in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) in Beijing, China. METHODS: Patients with AECOPD were recruited from 11 general hospitals. Sputum specimens were cultured and bacteria identified. Antibiotic susceptibility was determined for each isolate, and presence of antibiotic resistance genes was evaluated using polymerase chain reaction. RESULTS: Pathogenic bacteria were isolated from 109/318 patients (34.28%); 124 isolates of 22 pathogenic bacterial species were identified, including Klebsiella pneumoniae (16.94%), Pseudomonas aeruginosa (16.94%), Acinetobacter baumannii (11.29%), Streptococcus pneumoniae (8.87%), and Staphylococcus aureus (7.26%). S. aureus was sensitive to tigecycline, teicoplanin, vancomycin and linezolid but resistant to penicillin and levofloxacin. K.pneumoniae, P. aeruginosa, A. baumannii and E. coli were susceptible to amikacin and cefoperazone. CONCLUSIONS: K. pneumoniae and P. aeruginosa are the most common pathogenic bacteria in AECOPD cases in Beijing, China. Our antibiotic resistance findings may be helpful in selecting antibiotic therapy.
OBJECTIVE: A prospective observational study to investigate the distribution and antimicrobial resistance of pathogenic bacteria in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) in Beijing, China. METHODS:Patients with AECOPD were recruited from 11 general hospitals. Sputum specimens were cultured and bacteria identified. Antibiotic susceptibility was determined for each isolate, and presence of antibiotic resistance genes was evaluated using polymerase chain reaction. RESULTS: Pathogenic bacteria were isolated from 109/318 patients (34.28%); 124 isolates of 22 pathogenic bacterial species were identified, including Klebsiella pneumoniae (16.94%), Pseudomonas aeruginosa (16.94%), Acinetobacter baumannii (11.29%), Streptococcus pneumoniae (8.87%), and Staphylococcus aureus (7.26%). S. aureus was sensitive to tigecycline, teicoplanin, vancomycin and linezolid but resistant to penicillin and levofloxacin. K.pneumoniae, P. aeruginosa, A. baumannii and E. coli were susceptible to amikacin and cefoperazone. CONCLUSIONS:K. pneumoniae and P. aeruginosa are the most common pathogenic bacteria in AECOPD cases in Beijing, China. Our antibiotic resistance findings may be helpful in selecting antibiotic therapy.