Cecilia Bozzetti1, Rita Nizzoli1, Marcello Tiseo1, Anna Squadrilli1, Costanza Lagrasta2, Sebastiano Buti1, Donatello Gasparro1, Daniele Zanoni1, Maria Majori3, Massimo De Filippo4, Francesca Mazzoni5, Cristina Maddau6, Nadia Naldi1, Gabriella Sammarelli7, Caterina Frati8, Carmine Pinto1, Andrea Ardizzoni9. 1. Medical Oncology Unit, University Hospital of Parma, Parma, Italy. 2. Department of Biomedical, Biotechnological and Translational Sciences, University Hospital of Parma, Parma, Italy. 3. Pneumology Unit, University Hospital of Parma, Parma, Italy. 4. Radiology Unit, University Hospital of Parma, Parma, Italy. 5. Department of Medical Oncology, Careggi Hospital of Firenze, Firenze, Italy. 6. Cytopathology Unit, Cancer Prevention and Research Institute, Firenze, Italy. 7. Department of Clinical and Experimental Medicine, Haematology and Bone Marrow Transplantation Unit, University Hospital of Parma, Parma, Italy. 8. Clinical and Experimental Medicine, University Hospital of Parma, Parma, Italy. 9. Medical Oncology Unit, S.Orsola-Malpighi Hospital, Bologna, Italy.
Abstract
BACKGROUND: The identification of ALK and ROS1 rearrangements and the availability of an effective target therapy, such as crizotinib, represent a new option in the treatment of advanced non-small cell lung cancer (NSCLC) patients. In light of recent advances in non-invasive diagnostic procedures, we aimed to demonstrate that direct cytological smears are suitable for assessing ALK and ROS1 rearrangements in patients with NSCLC. METHODS: Fifty-five patients with a cytological diagnosis of lung adenocarcinoma (ADC) were evaluated for ALK rearrangements by fluorescence in situ hybridization (FISH) and 12 patients for ROS1 FISH rearrangements. Seventeen of the 55 cytological samples tested for ALK were obtained from the primary tumor and 38 from metastatic lesions. Ten of 12 samples evaluated for ROS1 were obtained from metastatic sites and two from the primary tumor. RESULTS: ALK FISH was successful in 49/55 (89%) cytological ADC samples and ROS1 FISH in all 12 cytological samples. ALK rearrangements were found in 3/13 (23%) primary tumors and 7/36 (19%) metastatic sites. ROS1 rearrangements were found in one of the two primary tumors and in two of the 10 metastases. Two of the three rearranged cases were tested on cytology after knowing that they were rearranged on histology in order to increase representativeness of ROS1 rearranged cases in this study. CONCLUSION: Whenever cytology represents the only available material for diagnosis and biological characterization of NSCLC, minimally invasive procedures may provide an additional important source of cellular material for FISH assessment of ALK and ROS1 rearrangements.
BACKGROUND: The identification of ALK and ROS1 rearrangements and the availability of an effective target therapy, such as crizotinib, represent a new option in the treatment of advanced non-small cell lung cancer (NSCLC) patients. In light of recent advances in non-invasive diagnostic procedures, we aimed to demonstrate that direct cytological smears are suitable for assessing ALK and ROS1 rearrangements in patients with NSCLC. METHODS: Fifty-five patients with a cytological diagnosis of lung adenocarcinoma (ADC) were evaluated for ALK rearrangements by fluorescence in situ hybridization (FISH) and 12 patients for ROS1 FISH rearrangements. Seventeen of the 55 cytological samples tested for ALK were obtained from the primary tumor and 38 from metastatic lesions. Ten of 12 samples evaluated for ROS1 were obtained from metastatic sites and two from the primary tumor. RESULTS:ALK FISH was successful in 49/55 (89%) cytological ADC samples and ROS1 FISH in all 12 cytological samples. ALK rearrangements were found in 3/13 (23%) primary tumors and 7/36 (19%) metastatic sites. ROS1 rearrangements were found in one of the two primary tumors and in two of the 10 metastases. Two of the three rearranged cases were tested on cytology after knowing that they were rearranged on histology in order to increase representativeness of ROS1 rearranged cases in this study. CONCLUSION: Whenever cytology represents the only available material for diagnosis and biological characterization of NSCLC, minimally invasive procedures may provide an additional important source of cellular material for FISH assessment of ALK and ROS1 rearrangements.
Authors: Francesco Facchinetti; Marcello Tiseo; Massimo Di Maio; Paolo Graziano; Emilio Bria; Giulio Rossi; Silvia Novello Journal: Transl Lung Cancer Res Date: 2016-06
Authors: Giuseppe Mariniello; Maria De Liso; Camilla Russo; Walter Del Vecchio; Oreste De Divitiis; Federico Bruno; Nicola Maggialetti; Francesco Arrigoni; Luca Brunese; Ferdinando Caranci Journal: Acta Biomed Date: 2019-04-24
Authors: María Sánchez-Ares; José M Cameselle-Teijeiro; Sergio Vázquez-Estévez; Martín Lázaro-Quintela; Ángel Vázquez-Boquete; Francisco J Afonso-Afonso; Joaquín Casal-Rubio; Ana L González-Piñeiro; Yolanda Rico-Rodríguez; José L Fírvida-Pérez; Juan Ruíz-Bañobre; Elena Couso; Lucía Santomé; Raquel Pérez-Becerra; Rosario García-Campelo; Margarita Amenedo; Cristina Azpitarte-Raposeiras; José Antúnez; Ihab Abdulkader Journal: Oncol Lett Date: 2016-06-28 Impact factor: 2.967