Zhou Liu1, Xiaojian Yin2, Lingying Liu3, Hua Tao4, Haihong Zhou2, Guoda Ma4, Lili Cui4, You Li4, Shuyan Zhang5, Zhi'en Xu2, LiFen Yao6, Zhiyou Cai7, Bin Zhao1, Keshen Li4. 1. Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China; Department of Neurology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China. 2. Department of Neurology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China. 3. Department of Neurology, Chenzhou No. 1 People's Hospital, Chenzhou, China. 4. Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China. 5. Department of Neurology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China. 6. Department of Neurology, The First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China. 7. Department of Neurology, Renmin Hospital, Hubei University of Medicine, Hubei, China.
Abstract
BACKGROUND AND AIMS: Temporal lobe epilepsy (TLE) is a prevalent form of epilepsy. TLE contributes to the majority of drug resistant epilepsy (DRE) cases and is associated with genetic factors. Kelch-like ECH-associated protein 1 (KEAP1)/Nuclear erythroid 2-related factor 2 (known as NFE2L2 or Nrf2) association has been implicated in neuroprotection due to induction of antioxidant enzymes. The association of one single KEAP1 gene nucleotide polymorphism (SNP) and nine NFE2L2 gene SNPs with TLE and DRE were examined to determine whether these SNPs influenced the risk of TLE and DRE in a Han population. SUBJECTS AND METHODS: A total of 184 TLE patients (including 72 DRE patients) and 183 controls were included in this analysis. The SNaPshot Multiplex kit was used to assess the genotypes. RESULTS: A NFE2L2 gene haplotype was identified as a risk factor for TLE (OR=7.11, 95% CI 1.53-32.98). Additionally, rs2706110 G>A in the NFE2L2 gene and rs1048290 C>G in the KEAP1 gene showed a significant risk for and a protective effect against DRE, respectively. CONCLUSION: Our findings suggest that variations in NFE2L2 gene increase the risk of TLE and DRE but that variations in KEAP1 gene play a protective role for DRE.
BACKGROUND AND AIMS: Temporal lobe epilepsy (TLE) is a prevalent form of epilepsy. TLE contributes to the majority of drug resistant epilepsy (DRE) cases and is associated with genetic factors. Kelch-like ECH-associated protein 1 (KEAP1)/Nuclear erythroid 2-related factor 2 (known as NFE2L2 or Nrf2) association has been implicated in neuroprotection due to induction of antioxidant enzymes. The association of one single KEAP1 gene nucleotide polymorphism (SNP) and nine NFE2L2 gene SNPs with TLE and DRE were examined to determine whether these SNPs influenced the risk of TLE and DRE in a Han population. SUBJECTS AND METHODS: A total of 184 TLEpatients (including 72 DRE patients) and 183 controls were included in this analysis. The SNaPshot Multiplex kit was used to assess the genotypes. RESULTS: A NFE2L2 gene haplotype was identified as a risk factor for TLE (OR=7.11, 95% CI 1.53-32.98). Additionally, rs2706110 G>A in the NFE2L2 gene and rs1048290 C>G in the KEAP1 gene showed a significant risk for and a protective effect against DRE, respectively. CONCLUSION: Our findings suggest that variations in NFE2L2 gene increase the risk of TLE and DRE but that variations in KEAP1 gene play a protective role for DRE.