| Literature DB >> 26147981 |
Corina M Ciocan, Elena Cubero-Leon, Christophe Minier, Jeanette M Rotchell.
Abstract
Entities:
Year: 2015 PMID: 26147981 PMCID: PMC4492971 DOI: 10.1371/journal.pone.0132080
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 1Real-time quantitative RT-PCR validation of differential screening results of M. edulis developing gonad versus mature gonad samples (1A–1E) and M. edulis experimentally-exposed to E2 (1F–1H).
Data plotted as mean±SEM, n = 15 samples. * = p<0.05; ** = p<0.01.
Differentially expressed (subtracted) mRNAs identified in M. edulis testis at two stages of gonadal development.
aDown-regulated in early developing testes relative to mature testes. bUp-regulated in early developing testes relative to mature testes.
| Clone accession no. | Category & gene identity | Length (bp) | Homolog species / Accession no. |
|
|---|---|---|---|---|
| HQ690234 | aSenescence-associated protein | 155 |
| 5.0 |
| AJ492924.1 | aHistone 2A | 301 |
| 1.0 |
| AY484747.1 | a16S ribosomal protein | 931 |
| 0 |
| FM995162.1 | aVitelline coat lysin M7 precursor | 635 |
| 3.0 |
| HQ678182 | aSialic acid binding lectin | 397 |
| 4.0 |
| CAX33833 | aPutative vitelline envelope receptor for lysin (VERL) | 663 |
| 4.0E-125 |
| HQ678180 | bTestis-specific serine/threonine kinase 1 (TSTK1) | 815 |
| 4.0 |
| HQ678181 | bTestis-specific A-kinase-anchoring-protein | 182 |
| 9.0 |
| HQ67816 | bHistone H2A isoform 2 | 332 |
| 1.0 |
| HQ678184 | bBeta-tubulin | 511 |
| 9.0 |
| AB257133 | bER | 111 |
| 1.0 |
| HQ678183 | bBindin precursor 5 repeat variant (acrosomal protein) | 392 |
| 7.0 |
| HQ678185 | bPhosphodiesterase 1 | 533 |
| 5.0 |
| AY130198.1 | bCytochrome c oxidase subunit III | 254 |
| 2.0 |
Differentially expressed (subtracted) mRNAs identified in M. edulis testis following E2 exposure.
aDown-regulated in control testes relative to E2-exposed testes. bUp-regulated in control testes relative to E2-exposed testes.
| Clone Accession No. | Category & gene identity (BlastX) | Length (bp) | Homolog species/Accession no. |
| |
|---|---|---|---|---|---|
| HQ664951 | aComplement C1q-like protein | 111 |
| XP_002918680.1 | 7.0 |
| HQ690237 | aAlpha tubulin | 297 |
| BAD80736.1 | 2.0 |
| HQ690238 | aBeta tubulin | 501 |
| NP_954525.1 | 2.0 |
| HQ690235 | aRibosomal protein L7 | 240 |
| AJ557884.1 | 3.0 |
| HQ690239 | aBromodomain adjacent to zinc finger domain, 1A | 369 |
| XP_426440.2 | 7.0 |
| HQ690240 | aElongation factor 1 gamma | 162 |
| NP_001171816.1 | 1.0 |
| HQ664949 | aVitelline envelope zona pellucida domain 9 | 900 |
| ABE72949.1 | 1.0 |
| HQ664950 | aC1q domain containing protein | 141 |
| ADD17343 | 7.0 |
| HQ664948 | aRWD domain containing protein 4A | 240 |
| ACO11028.1 | 3.0 |
| HQ664952 | aHemaglutinin/amoebocyte aggregation factor precursor | 240 |
| ACI68653.1 | 1.0 |
| YP_073337 | aCytochrome c oxidase subunit II | 448 |
| YP_073337.1 | 4.0 |
| YP_073338.1 | aNADH dehydrogenase subunit 1 | 647 |
| YP_073338.1 | 4.0 |
| HQ690236 | aTriosephosphate isomerase TIM | 156 |
| AAP79983.1 | 3.0 |
| CAX33833 | aPutative vitelline envelope receptor for lysin (VERL) | 663 |
| CAX33833 | 4.0E-125 |
| AAV68423 | bCytochrome c oxidase subunit 1 | 534 |
| AAV68411.1 | 8.0 |
| AAV68416 | bCytochrome b | 302 |
| AAV68404.1 | 6.0 |
| HQ690243 | bFerritin-like protein | 492 |
| AAQ12076.1 | 2.0 |
| HQ690241 | bSenescence-associated protein | 318 |
| XP_002118266.1 | 6.0 |
| HQ690244 | bSpectrin beta chain | 293 |
| EFN75523.1 | 1.0 |