Yan Li1, Hongmei Guo1, Xijun Wang1, Yang Lu2, Chunyu Yang3, Pishan Yang4. 1. School of Stomatology, Shandong University, Jinan, PR China; Shandong provincial key laboratory of oral tissue regeneration, Jinan, PR China. 2. Jining Medical University. 3. Weifang Medical University. 4. School of Stomatology, Shandong University, Jinan, PR China; Shandong provincial key laboratory of oral tissue regeneration, Jinan, PR China. Electronic address: yangps@sdu.edu.cn.
Abstract
OBJECTIVE: This study was conducted to investigate effects of coinfection of Porphyromonas gingivalis (P. gingivalis) or Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) with Fusobacterium nucleatum (F. nucleatum) on their adhering and invasive capacity to human gingival epithelial cells as well as the expression of interleukin-8 (IL-8) and human beta-defensin-2 (hBD-2) in human gingival epithelial cells. DESIGN: P. gingivalis and A. actinomycetemcomitans were tested for their ability to attach and invade a human gingival epithelial cell line (Ca9-22) alone or coinfecting with F. nucleatum. Also, expression levels of IL-8 and hBD-2 were detected respectively using enzyme linked immunosorbent assay (ELISA) and real-time reverse transcription PCR (RT-PCR) when Ca9-22 cells were infected with P. gingivalis and A. actinomycetemcomitans alone or coinfecting with F. nucleatum. RESULTS: F. nucleatum, P. gingivalis and A. actinomycetemcomitans were allowed to adhere and invade Ca9-22 cells, either each strain alone or under coinfection. The adhering and invasive abilities of P. gingivalis and A. actinomycetemcomitans were significantly greater when they were coincubated with F. nucleatum (P<0.05) than either of them alone. These enhancements were inhibited by galactose. In addition, P. gingivalis and A. actinomycetemcomitans inhibited the activation of IL-8 and hBD-2 by F. nucleatum. Also, galactose disrupted this inhibition on the expression of IL-8 and hBD-2. CONCLUSION: These results suggested coinfection with F. nucleatum can enhance adhesion and invasion of P. gingivalis and A. actinomycetemcomitans to Ca9-22 cells, as well as inhibition on host innate immune response.
OBJECTIVE: This study was conducted to investigate effects of coinfection of Porphyromonas gingivalis (P. gingivalis) or Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) with Fusobacterium nucleatum (F. nucleatum) on their adhering and invasive capacity to human gingival epithelial cells as well as the expression of interleukin-8 (IL-8) and humanbeta-defensin-2 (hBD-2) in human gingival epithelial cells. DESIGN:P. gingivalis and A. actinomycetemcomitans were tested for their ability to attach and invade a human gingival epithelial cell line (Ca9-22) alone or coinfecting with F. nucleatum. Also, expression levels of IL-8 and hBD-2 were detected respectively using enzyme linked immunosorbent assay (ELISA) and real-time reverse transcription PCR (RT-PCR) when Ca9-22 cells were infected with P. gingivalis and A. actinomycetemcomitans alone or coinfecting with F. nucleatum. RESULTS:F. nucleatum, P. gingivalis and A. actinomycetemcomitans were allowed to adhere and invade Ca9-22 cells, either each strain alone or under coinfection. The adhering and invasive abilities of P. gingivalis and A. actinomycetemcomitans were significantly greater when they were coincubated with F. nucleatum (P<0.05) than either of them alone. These enhancements were inhibited by galactose. In addition, P. gingivalis and A. actinomycetemcomitans inhibited the activation of IL-8 and hBD-2 by F. nucleatum. Also, galactose disrupted this inhibition on the expression of IL-8 and hBD-2. CONCLUSION: These results suggested coinfection with F. nucleatum can enhance adhesion and invasion of P. gingivalis and A. actinomycetemcomitans to Ca9-22 cells, as well as inhibition on host innate immune response.
Authors: John D Perpich; Lan Yakoumatos; Kendall S Stocke; Gina R Lewin; Anayancy Ramos; Deborah R Yoder-Himes; Marvin Whiteley; Richard J Lamont Journal: Infect Immun Date: 2022-05-16 Impact factor: 3.609
Authors: Charles M Cobb; Patricia J Kelly; Karen B Williams; Shilpa Babbar; Mubashir Angolkar; Richard J Derman Journal: Int J Womens Health Date: 2017-08-08