Yangyang Xu1, Jie Zhang1, Wanglin Jiang2, Shuping Zhang3. 1. School of Pharmaceutical Sciences, Binzhou Medical University, Yantai, 264003, PR. China. 2. School of Pharmaceutical Sciences, Binzhou Medical University, Yantai, 264003, PR. China. Electronic address: jwl518@163.com. 3. School of Pharmaceutical Sciences, Binzhou Medical University, Yantai, 264003, PR. China. Electronic address: zsp1963yt@163.com.
Abstract
OBJECTIVE: In the present study, we sought to elucidate whether astaxanthin contributes to induce angiogenesis and its mechanisms. MATERIALS AND METHODS: To this end, we examined the role of astaxanthin on human brain microvascular endothelial cell line (HBMEC) and rat aortic smooth muscle cell (RASMC) proliferation, invasion and tube formation in vitro. For study of mechanism, the Wnt/β-catenin signaling pathway inhibitor IWR-1-endo was used. HMBECs and RASMCs proliferation were tested by cell counting. Scratch adhesion test was used to assess the ability of invasion. A matrigel tube formation assay was performed to test capillary tube formation ability. The Wnt/β-catenin pathway activation in HMBECs and RASMCs were tested by Western blot. RESULTS: Our data suggested that astaxanthin induces angiogenesis by increasing proliferation, invasion and tube formation in vitro. Wnt and β-catenin expression were increased by astaxanthin and counteracted by IWR-1-endo in HMBECs and RASMCs. Tube formation was increased by astaxanthin and counteracted by IWR-1-endo. CONCLUSIONS: It may be suggested that astaxanthin induces angiogenesis in vitro via a programmed Wnt/β-catenin signaling pathway.
OBJECTIVE: In the present study, we sought to elucidate whether astaxanthin contributes to induce angiogenesis and its mechanisms. MATERIALS AND METHODS: To this end, we examined the role of astaxanthin on human brain microvascular endothelial cell line (HBMEC) and rat aortic smooth muscle cell (RASMC) proliferation, invasion and tube formation in vitro. For study of mechanism, the Wnt/β-catenin signaling pathway inhibitor IWR-1-endo was used. HMBECs and RASMCs proliferation were tested by cell counting. Scratch adhesion test was used to assess the ability of invasion. A matrigel tube formation assay was performed to test capillary tube formation ability. The Wnt/β-catenin pathway activation in HMBECs and RASMCs were tested by Western blot. RESULTS: Our data suggested that astaxanthin induces angiogenesis by increasing proliferation, invasion and tube formation in vitro. Wnt and β-catenin expression were increased by astaxanthin and counteracted by IWR-1-endo in HMBECs and RASMCs. Tube formation was increased by astaxanthin and counteracted by IWR-1-endo. CONCLUSIONS: It may be suggested that astaxanthin induces angiogenesis in vitro via a programmed Wnt/β-catenin signaling pathway.
Authors: Yu Chiuan Wu; Han Hsiang Huang; Yi Jhen Wu; Ioannis Manousakas; Chin Chang Yang; Shyh Ming Kuo Journal: Int J Mol Sci Date: 2019-08-20 Impact factor: 5.923
Authors: Eric M Thompson; Stephen T Keir; Talaignair Venkatraman; Christopher Lascola; Kristen W Yeom; Andrew B Nixon; Yingmiao Liu; Daniel Picard; Marc Remke; Darell D Bigner; Vijay Ramaswamy; Michael D Taylor Journal: Neuro Oncol Date: 2017-09-01 Impact factor: 12.300