Wei Peng1, Changling Hu2, Zhiheng Shu3, Ting Han3, Luping Qin4, Chengjian Zheng5. 1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, P.R. China; College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, P.R. China. 2. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, P.R. China; Department of Natural Products Chemistry, School of Pharmacy, Fudan University, Shanghai 201203, P.R. China. 3. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, P.R. China. 4. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, P.R. China. Electronic address: qinsmmu@126.com. 5. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, P.R. China. Electronic address: zhengchengjian@smmu.edu.cn.
Abstract
BACKGROUND: Fagopyrum tataricum (L.) Gaertn is a famous drinking food and herbal medicine in China, and have been commonly used for treating various diseases. PURPOSE: This study was designed to investigate the antitumor effect of tatariside F (TF) isolated from the roots of F. tataricum against H22 hepatocellular carcinoma (HCC) in vitro and in vivo and explore the possible mechanisms. METHODS: In our present study, the anti-proliferative effect of TF against H22 cells was evaluated by MTT method. Furthermore, a mice xenograft model was established to investigate the antitumor effect of TF on HCC in vivo, and the possible mechanisms were determined by western blot and fluorescence polarization binding assay. In addition, the protective effect of TF on liver was also investigated by examining the histopathological changes and determining the liver biochemical parameters. RESULTS: Our results demonstrated that TF possessed notable antitumor effect against HCC both in vivo and in vitro, and the possible mechanism might be related to up-regulation of the protein expressions of Bax and p53, and down-regulation of Bcl-2. What's more, TF also exhibited protective effects against CTX (cyclophosphamide)-induced liver damages when co-administrated with CTX. CONCLUSION: Our study suggested that TF possess notable antitumor effects against HCC and might play a favorable role in drug combination therapy against tumors with protective effect on liver.
BACKGROUND:Fagopyrum tataricum (L.) Gaertn is a famous drinking food and herbal medicine in China, and have been commonly used for treating various diseases. PURPOSE: This study was designed to investigate the antitumor effect of tatariside F (TF) isolated from the roots of F. tataricum against H22 hepatocellular carcinoma (HCC) in vitro and in vivo and explore the possible mechanisms. METHODS: In our present study, the anti-proliferative effect of TF against H22 cells was evaluated by MTT method. Furthermore, a mice xenograft model was established to investigate the antitumor effect of TF on HCC in vivo, and the possible mechanisms were determined by western blot and fluorescence polarization binding assay. In addition, the protective effect of TF on liver was also investigated by examining the histopathological changes and determining the liver biochemical parameters. RESULTS: Our results demonstrated that TF possessed notable antitumor effect against HCC both in vivo and in vitro, and the possible mechanism might be related to up-regulation of the protein expressions of Bax and p53, and down-regulation of Bcl-2. What's more, TF also exhibited protective effects against CTX (cyclophosphamide)-induced liver damages when co-administrated with CTX. CONCLUSION: Our study suggested that TF possess notable antitumor effects against HCC and might play a favorable role in drug combination therapy against tumors with protective effect on liver.
Authors: Qing Zhang; Wen Wen Chen; Xue Sun; Die Qian; Dan Dan Tang; Li Lin Zhang; Mei Yan Li; Lin Yu Wang; Chun-Jie Wu; Wei Peng Journal: Int J Biol Sci Date: 2022-05-16 Impact factor: 10.750