Literature DB >> 26141312

Skin Biopsy and Patient-Specific Stem Cell Lines.

Yao Li1,2, Huy V Nguyen3, Stephen H Tsang4,5,6.   

Abstract

The generation of patient-specific induced pluripotent stem (iPS) cells permits the development of next-generation patient-specific systems biology models reflecting personalized genomics profiles to better understand pathophysiology. In this chapter, we describe how to create a patient-specific iPS cell line. There are three major steps: (1) performing a skin biopsy procedure on the patient; (2) extracting human fibroblast cells from the skin biopsy tissue; and (3) reprogramming patient-specific fibroblast cells into the pluripotent stem cell stage.

Entities:  

Keywords:  Fibroblast; Human iPS; Reprogramming; Sendai virus; Skin biopsy

Mesh：

Substances：

Year:  2016        PMID： 26141312      PMCID： PMC4836060          DOI： 10.1007/7651_2015_225

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Isolation of a virus from a sporadic case of Izumi fever.

Authors:  M KUROYA; Y YOSHINARI; N ISHIDA; K NODA; E KOSEKI
Journal:  Tohoku J Exp Med       Date:  1953-06       Impact factor: 1.848

2.  Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.

Authors:  Kazutoshi Takahashi; Shinya Yamanaka
Journal:  Cell       Date:  2006-08-10       Impact factor: 41.582

3.  A cytoplasmic RNA vector derived from nontransmissible Sendai virus with efficient gene transfer and expression.

Authors:  H O Li; Y F Zhu; M Asakawa; H Kuma; T Hirata; Y Ueda; Y S Lee; M Fukumura; A Iida; A Kato; Y Nagai; M Hasegawa
Journal:  J Virol       Date:  2000-07       Impact factor: 5.103

4.  Generation of human-induced pluripotent stem cells.

Authors:  In-Hyun Park; Paul H Lerou; Rui Zhao; Hongguang Huo; George Q Daley
Journal:  Nat Protoc       Date:  2008       Impact factor: 13.491

5.  Validation of genome-wide association study (GWAS)-identified disease risk alleles with patient-specific stem cell lines.

Authors:  Jin Yang; Yao Li; Lawrence Chan; Yi-Ting Tsai; Wen-Hsuan Wu; Huy V Nguyen; Chun-Wei Hsu; Xiaorong Li; Lewis M Brown; Dieter Egli; Janet R Sparrow; Stephen H Tsang
Journal:  Hum Mol Genet       Date:  2014-02-04       Impact factor: 6.150

6.  Gene therapy in patient-specific stem cell lines and a preclinical model of retinitis pigmentosa with membrane frizzled-related protein defects.

Authors:  Yao Li; Wen-Hsuan Wu; Chun-Wei Hsu; Huy V Nguyen; Yi-Ting Tsai; Lawrence Chan; Takayuki Nagasaki; Irene H Maumenee; Lawrence A Yannuzzi; Quan V Hoang; Haiqing Hua; Dieter Egli; Stephen H Tsang
Journal:  Mol Ther       Date:  2014-06-04       Impact factor: 11.454

7.  Generation of germline-competent induced pluripotent stem cells.

Authors:  Keisuke Okita; Tomoko Ichisaka; Shinya Yamanaka
Journal:  Nature       Date:  2007-06-06       Impact factor: 49.962

8.  iPS cell modeling of Best disease: insights into the pathophysiology of an inherited macular degeneration.

Authors:  Ruchira Singh; Wei Shen; David Kuai; Jessica M Martin; Xiangrong Guo; Molly A Smith; Enio T Perez; M Joseph Phillips; Joseph M Simonett; Kyle A Wallace; Amelia D Verhoeven; Elizabeth E Capowski; Xiaoqing Zhang; Yingnan Yin; Patrick J Halbach; Gerald A Fishman; Lynda S Wright; Bikash R Pattnaik; David M Gamm
Journal:  Hum Mol Genet       Date:  2012-11-08       Impact factor: 6.150

9.  Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome.

Authors:  Noemi Fusaki; Hiroshi Ban; Akiyo Nishiyama; Koichi Saeki; Mamoru Hasegawa
Journal:  Proc Jpn Acad Ser B Phys Biol Sci       Date:  2009       Impact factor: 3.493
  9 in total
  7 in total

1.  Differentiation, Maintenance, and Analysis of Human Retinal Pigment Epithelium Cells: A Disease-in-a-dish Model for BEST1 Mutations.

Authors:  Alec Kittredge; Changyi Ji; Yu Zhang; Tingting Yang
Journal:  J Vis Exp       Date:  2018-08-24       Impact factor: 1.355

2.  Creating Cell Model 2.0 Using Patient Samples Carrying a Pathogenic Mitochondrial DNA Mutation: iPSC Approach for LHON.

Authors:  Pragya Singh; Tyler Bahr; Xiaoxu Zhao; Peiqing Hu; Marcel Daadi; TaoSheng Huang; Yidong Bai
Journal:  Methods Mol Biol       Date:  2022

3.  Patient-specific mutations impair BESTROPHIN1's essential role in mediating Ca2+-dependent Cl- currents in human RPE.

Authors:  Yao Li; Yu Zhang; Yu Xu; Alec Kittredge; Nancy Ward; Shoudeng Chen; Stephen H Tsang; Tingting Yang
Journal:  Elife       Date:  2017-10-24       Impact factor: 8.140

Review 4.  CRISPR-Cas Genome Surgery in Ophthalmology.

Authors:  James E DiCarlo; Jesse D Sengillo; Sally Justus; Thiago Cabral; Stephen H Tsang; Vinit B Mahajan
Journal:  Transl Vis Sci Technol       Date:  2017-05-31       Impact factor: 3.283

5.  Investigation and Restoration of BEST1 Activity in Patient-derived RPEs with Dominant Mutations.

Authors:  Changyi Ji; Yao Li; Alec Kittredge; Austin Hopiavuori; Nancy Ward; Peng Yao; Yohta Fukuda; Yu Zhang; Stephen H Tsang; Tingting Yang
Journal:  Sci Rep       Date:  2019-12-13       Impact factor: 4.379

6.  Human Cardiac Organoids for Modeling Genetic Cardiomyopathy.

Authors:  Michele Filippo Buono; Lisa von Boehmer; Jaan Strang; Simon P Hoerstrup; Maximilian Y Emmert; Bramasta Nugraha
Journal:  Cells       Date:  2020-07-20       Impact factor: 6.600

7.  Distinct expression requirements and rescue strategies for BEST1 loss- and gain-of-function mutations.

Authors:  Qingqing Zhao; Yang Kong; Alec Kittredge; Yao Li; Yin Shen; Yu Zhang; Stephen H Tsang; Tingting Yang
Journal:  Elife       Date:  2021-06-01       Impact factor: 8.140
  7 in total

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