Guoguang Ma1, Bin Shi2, Jingquan Liu1, Hongze Zhang1, Zijun YinTao1, Xiaoli Lou3, Dongyu Liang3, Yanqiang Hou3, Shengxia Wan1, Wanhua Yang1. 1. Department of General Intensive Care Unit, Songjiang Hospital Affiliated to First People's Hospital, Shanghai Jiao Tong University, No. 746, Zhongshan Road, Shanghai, 201600, China. 2. Department of General Intensive Care Unit, Songjiang Hospital Affiliated to First People's Hospital, Shanghai Jiao Tong University, No. 746, Zhongshan Road, Shanghai, 201600, China. 18918288036@163.com. 3. Department of Central Laboratory, Songjiang Hospital Affiliated to First People's Hospital, Shanghai Jiao Tong University, Shanghai, 201600, China.
Abstract
BACKGROUND AND AIMS: PepT1 can transport bacterial oligopeptide products and induce intestinal inflammation. Our aim was to investigate the mechanism of the small intestine injury induced by bacterial oligopeptide product muramyl dipeptide (MDP) which is transported by PepT1. METHODS: We perfused the jejunum with a solution with or without MDP, or with a solution of MDP + Gly-Gly and explored the degree of inflammation to determine the role of PepT1-Nod2 signaling pathway in small intestine mucosa. RESULTS: MDP perfusion induced inflammatory cell accumulation and intestinal damage, accompanied by an increase in mucosal Nod2 and Rip2 transcript expression. NFκB activity and inflammatory cytokine expression, including serum levels of TNF-α, IL-1β, and IL-6, increased in the MDP group compared to the controls; these effects were reversed by perfusion of the nutritional dipeptide Gly-Gly. CONCLUSION: MDP can be transported through PepT1, causing inflammatory damage in the rat small intestine. Nod2-Rip2-NFκB signaling involved in the small intestinal inflammatory injury caused by MDP which is transported through PepT1.
BACKGROUND AND AIMS: PepT1 can transport bacterial oligopeptide products and induce intestinal inflammation. Our aim was to investigate the mechanism of the small intestine injury induced by bacterial oligopeptide product muramyl dipeptide (MDP) which is transported by PepT1. METHODS: We perfused the jejunum with a solution with or without MDP, or with a solution of MDP + Gly-Gly and explored the degree of inflammation to determine the role of PepT1-Nod2 signaling pathway in small intestine mucosa. RESULTS:MDP perfusion induced inflammatory cell accumulation and intestinal damage, accompanied by an increase in mucosal Nod2 and Rip2 transcript expression. NFκB activity and inflammatory cytokine expression, including serum levels of TNF-α, IL-1β, and IL-6, increased in the MDP group compared to the controls; these effects were reversed by perfusion of the nutritional dipeptideGly-Gly. CONCLUSION:MDP can be transported through PepT1, causing inflammatory damage in the rat small intestine. Nod2-Rip2-NFκB signaling involved in the small intestinal inflammatory injury caused by MDP which is transported through PepT1.
Authors: Stephan R Vavricka; Mark W Musch; Jonathan E Chang; Yasushi Nakagawa; Kittiporn Phanvijhitsiri; Tonya S Waypa; Didier Merlin; Olaf Schneewind; Eugene B Chang Journal: Gastroenterology Date: 2004-11 Impact factor: 22.682
Authors: Stephen E Girardin; Ivo G Boneca; Jérôme Viala; Mathias Chamaillard; Agnès Labigne; Gilles Thomas; Dana J Philpott; Philippe J Sansonetti Journal: J Biol Chem Date: 2003-01-13 Impact factor: 5.157
Authors: M Buyse; F Berlioz; S Guilmeau; A Tsocas; T Voisin; G Péranzi; D Merlin; M Laburthe; M J Lewin; C Rozé; A Bado Journal: J Clin Invest Date: 2001-11 Impact factor: 14.808