N Ardjomandi1, F Duttenhoefer2, S Xavier3, T Oshima4, A Kuenz2, S Sauerbier5. 1. Department of Oral- and Maxillofacial Surgery, University Hospital Tuebingen, Osianderstr. 2-8, 72076 Tuebingen, Germany. Electronic address: N.ardjomandi@gmail.com. 2. Department of Oral and Craniomaxillofacial Surgery, University Medical Center Freiburg, Freiburg, Germany. 3. Department of Oral and Maxillofacial Surgery, University of Sao Paulo, Faculty of Dentistry, Ribeirao Preto, Sao Paulo, Brazil. 4. Department of Oral and Craniomaxillofacial Surgery, University Medical Center Freiburg, Freiburg, Germany; Faculty of Health Sciences, Institute for International Cooperation, Okayama, Japan. 5. Department of Oral- and Maxillofacial Surgery, University Hospital Tuebingen, Osianderstr. 2-8, 72076 Tuebingen, Germany; Department of Oral and Craniomaxillofacial Surgery, University Medical Center Freiburg, Freiburg, Germany.
Abstract
INTRODUCTION: The aim of this work was to analyse the suitability of mesenchymal stem cell isolation by FICOLL density centrifugation and the closed bone marrow aspirate concentrate (BMAC) system for sinus augmentation with bovine bone mineral (BBM) in the sheep model. METHODS: 16 sheep underwent sinus augmentation with BBM and MSCs; they were divided between two groups with survival points of 8 and 16 weeks. For the FICOLL control arm three, and for the BMAC test arm, five augmentations were performed for each time point. The derived cell numbers were counted; a colony forming unit (CFU) assay was performed; the pluripotency of the MSCs was proved; histological and histomorphometrical analysis were performed. RESULTS: The approach of using BBM and MSCs in combination with fibrin adhesive was sufficient for new bone formation as the FICOLL experiment indicated. However, due to significantly lower cell numbers isolated using the BMAC in sheep, less new bone was formed in the test arm. CONCLUSIONS: The BMAC system is well suited for human MSC isolation but it needs to be optimized to fit sheep cell characteristics if it is to be used in this animal model.
INTRODUCTION: The aim of this work was to analyse the suitability of mesenchymal stem cell isolation by FICOLL density centrifugation and the closed bone marrow aspirate concentrate (BMAC) system for sinus augmentation with bovine bone mineral (BBM) in the sheep model. METHODS: 16 sheep underwent sinus augmentation with BBM and MSCs; they were divided between two groups with survival points of 8 and 16 weeks. For the FICOLL control arm three, and for the BMAC test arm, five augmentations were performed for each time point. The derived cell numbers were counted; a colony forming unit (CFU) assay was performed; the pluripotency of the MSCs was proved; histological and histomorphometrical analysis were performed. RESULTS: The approach of using BBM and MSCs in combination with fibrin adhesive was sufficient for new bone formation as the FICOLL experiment indicated. However, due to significantly lower cell numbers isolated using the BMAC in sheep, less new bone was formed in the test arm. CONCLUSIONS: The BMAC system is well suited for human MSC isolation but it needs to be optimized to fit sheep cell characteristics if it is to be used in this animal model.
Authors: Olli-Pekka Lappalainen; Sakari Karhula; Marianne Haapea; Laura Kyllönen; Suvi Haimi; Susanna Miettinen; Simo Saarakkala; Jarkko Korpi; Leena P Ylikontiola; Willy S Serlo; George K Sándor Journal: Childs Nerv Syst Date: 2016-01-19 Impact factor: 1.475
Authors: R Verboket; M Leiblein; C Seebach; C Nau; M Janko; M Bellen; H Bönig; D Henrich; I Marzi Journal: Eur J Trauma Emerg Surg Date: 2018-01-19 Impact factor: 3.693