Manon Lecomte1, Claire Bourlieu2, Emmanuelle Meugnier3, Armelle Penhoat1, David Cheillan4, Gaëlle Pineau1, Emmanuelle Loizon3, Michèle Trauchessec5, Mathilde Claude2, Olivia Ménard2, Alain Géloën1, Fabienne Laugerette1, Marie-Caroline Michalski6. 1. UMR 1397 National Institute for Agricultural Research (INRA), Lyon 1 University, U1060 National Institute of Health and Medical Research (INSERM), National Institute of Applied Science of Lyon, INSA-Lyon, Institute for Multidisciplinary Biochemistry of Lipids, Cardiovascular, Metabolism, Diabetologia and Nutrition Laboratory, Villeurbanne, France; 2. UMR 1253 National Institute for Agricultural Research (INRA), Science & Technology of Milk and Egg, Rennes, France; Agrocampus Ouest, Science & Technology of Milk and Egg, Rennes, France; 3. U1060 National Institute of Health and Medical Research (INSERM), UMR 1397 National Institute for Agricultural Research (INRA), Cardiovascular, Metabolism, Diabetologia and Nutrition Laboratory, Oullins, France; and. 4. UMR 1397 National Institute for Agricultural Research (INRA), Lyon 1 University, U1060 National Institute of Health and Medical Research (INSERM), National Institute of Applied Science of Lyon, INSA-Lyon, Institute for Multidisciplinary Biochemistry of Lipids, Cardiovascular, Metabolism, Diabetologia and Nutrition Laboratory, Villeurbanne, France; Hereditary Metabolic Diseases Department, East Medical Group, Lyon Civil Hospitals, Lyon, France. 5. Hereditary Metabolic Diseases Department, East Medical Group, Lyon Civil Hospitals, Lyon, France. 6. UMR 1397 National Institute for Agricultural Research (INRA), Lyon 1 University, U1060 National Institute of Health and Medical Research (INSERM), National Institute of Applied Science of Lyon, INSA-Lyon, Institute for Multidisciplinary Biochemistry of Lipids, Cardiovascular, Metabolism, Diabetologia and Nutrition Laboratory, Villeurbanne, France; marie-caroline.michalski@insa-lyon.fr.
Abstract
BACKGROUND: Polar lipid (PL) emulsifiers such as milk PLs (MPLs) may affect digestion and subsequent lipid metabolism, but focused studies on postprandial lipemia are lacking. OBJECTIVE: We evaluated the impact of MPLs on postprandial lipemia in mice and on lipid digestion in vitro. METHODS: Female Swiss mice were gavaged with 150 μL of an oil-in-water emulsion stabilized with 5.7 mg of either MPLs or soybean PLs (SPLs) and killed after 1, 2, or 4 h. Plasma lipids were quantified and in the small intestine, gene expression was analyzed by reverse transcriptase-quantitative polymerase chain reaction. Emulsions were lipolyzed in vitro using a static human digestion model; triglyceride (TG) disappearance was followed by thin-layer chromatography. RESULTS: In mice, after 1 h, plasma TGs tended to be higher in the MPL group than in the SPL group (141 μg/mL vs. 90 μg/mL; P = 0.07) and nonesterified fatty acids (NEFAs) were significantly higher (64 μg/mL vs. 44 μg/mL; P < 0.05). The opposite was observed after 4 h with lower TGs (21 μg/mL vs. 35 μg/mL; P < 0.01) and NEFAs (20 μg/mL vs. 32 μg/mL; P < 0.01) in the MPL group compared with the SPL group. This was associated at 4 h with a lower gene expression of apolipoprotein B (Apob) and Secretion Associated, Ras related GTPase 1 gene homolog B (Sar1b), in the duodenum of MPL mice compared with SPL mice (P < 0.05). In vitro, during the intestinal phase, TGs were hydrolyzed more in the MPL emulsion than in the SPL emulsion (decremental AUCs were 1750%/min vs. 180%/min; P < 0.01). MPLs enhance lipid intestinal hydrolysis and promote more rapid intestinal lipid absorption and sharper kinetics of lipemia. CONCLUSIONS: Postprandial lipemia in mice can be modulated by emulsifying with MPLs compared with SPLs, partly through differences in chylomicron assembly, and TG hydrolysis rate as observed in vitro. MPLs may thereby contribute to the long-term regulation of lipid metabolism.
BACKGROUND: Polar lipid (PL) emulsifiers such as milk PLs (MPLs) may affect digestion and subsequent lipid metabolism, but focused studies on postprandial lipemia are lacking. OBJECTIVE: We evaluated the impact of MPLs on postprandial lipemia in mice and on lipid digestion in vitro. METHODS: Female Swiss mice were gavaged with 150 μL of an oil-in-water emulsion stabilized with 5.7 mg of either MPLs or soybean PLs (SPLs) and killed after 1, 2, or 4 h. Plasma lipids were quantified and in the small intestine, gene expression was analyzed by reverse transcriptase-quantitative polymerase chain reaction. Emulsions were lipolyzed in vitro using a static human digestion model; triglyceride (TG) disappearance was followed by thin-layer chromatography. RESULTS: In mice, after 1 h, plasma TGs tended to be higher in the MPL group than in the SPL group (141 μg/mL vs. 90 μg/mL; P = 0.07) and nonesterified fatty acids (NEFAs) were significantly higher (64 μg/mL vs. 44 μg/mL; P < 0.05). The opposite was observed after 4 h with lower TGs (21 μg/mL vs. 35 μg/mL; P < 0.01) and NEFAs (20 μg/mL vs. 32 μg/mL; P < 0.01) in the MPL group compared with the SPL group. This was associated at 4 h with a lower gene expression of apolipoprotein B (Apob) and Secretion Associated, Ras related GTPase 1 gene homolog B (Sar1b), in the duodenum of MPLmice compared with SPLmice (P < 0.05). In vitro, during the intestinal phase, TGs were hydrolyzed more in the MPL emulsion than in the SPL emulsion (decremental AUCs were 1750%/min vs. 180%/min; P < 0.01). MPLs enhance lipid intestinal hydrolysis and promote more rapid intestinal lipid absorption and sharper kinetics of lipemia. CONCLUSIONS: Postprandial lipemia in mice can be modulated by emulsifying with MPLs compared with SPLs, partly through differences in chylomicron assembly, and TG hydrolysis rate as observed in vitro. MPLs may thereby contribute to the long-term regulation of lipid metabolism.
Authors: André Brodkorb; Lotti Egger; Marie Alminger; Paula Alvito; Ricardo Assunção; Simon Ballance; Torsten Bohn; Claire Bourlieu-Lacanal; Rachel Boutrou; Frédéric Carrière; Alfonso Clemente; Milena Corredig; Didier Dupont; Claire Dufour; Cathrina Edwards; Matt Golding; Sibel Karakaya; Bente Kirkhus; Steven Le Feunteun; Uri Lesmes; Adam Macierzanka; Alan R Mackie; Carla Martins; Sébastien Marze; David Julian McClements; Olivia Ménard; Mans Minekus; Reto Portmann; Cláudia N Santos; Isabelle Souchon; R Paul Singh; Gerd E Vegarud; Martin S J Wickham; Werner Weitschies; Isidra Recio Journal: Nat Protoc Date: 2019-03-18 Impact factor: 13.491
Authors: Ruisong Pei; Diana M DiMarco; Kelley K Putt; Derek A Martin; Chureeporn Chitchumroonchokchai; Richard S Bruno; Bradley W Bolling Journal: J Nutr Date: 2018-06-01 Impact factor: 4.798