Elena Anzivino1, Donatella Maria Rodio1, Monica Mischitelli1, Anna Bellizzi2, Alessandro Sciarra3, Stefano Salciccia3, Vincenzo Gentile3, Valeria Pietropaolo4. 1. Department of Health Sciences and Infectious Diseases, Sapienza University, Rome, Italy. 2. Department of Public Health and Infectious Diseases, Pasteur Institute, Cenci-Bolognetti Foundation, Sapienza University of Rome, Rome, Italy Department of Neuroscience, Temple University School of Medicine, Philadelphia, PA, U.S.A. 3. Department of Obstretics, Gynecology and Urological Sciences, Sapienza University, Rome, Italy. 4. Department of Health Sciences and Infectious Diseases, Sapienza University, Rome, Italy Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, PA, U.S.A. valeria.pietropaolo@uniroma1.it.
Abstract
BACKGROUND: Prostate cancer (PC) represents the most frequently diagnosed cancer in men. Exposure to infectious agents has been considered to induce prostatic inflammation and cancerous transformation. Controversial data exist concerning the role of the human polyomaviruses BK (BKV) and JC (JCV) in PC etiology. Therefore, a possible association between these polyomaviruses and PC was investigated. MATERIALS AND METHODS: Urine, blood and fresh prostatic tissue specimens were collected from 26 patients with PC. The presence of BKV and JCV, the possible non-coding control region (NCCR) variations and the genotyping analysis of viral protein 1 (VP1) of both viruses were assessed. RESULTS: Data showed a preferential viral re-activation in the urinary compartment and a statistically significant prevalence of JC viruria and of BKV in PC tissues. A BKV DDP-like NCCR sequence was isolated in two patients, whereas JCV NCCR was consistently of an archetypal structural organization. A prevalence of the European genotypes was observed for both viruses. CONCLUSION: Our data demonstrated the presence of JCV DNA in 14/24 (58.3%) cancerous prostatic tissue specimens, confirming the results obtained in a previous study, in which JCV has been defined as common inhabitant of the prostate, and opening the discussion about its potential role in PC. Copyright
BACKGROUND:Prostate cancer (PC) represents the most frequently diagnosed cancer in men. Exposure to infectious agents has been considered to induce prostatic inflammation and cancerous transformation. Controversial data exist concerning the role of the humanpolyomaviruses BK (BKV) and JC (JCV) in PC etiology. Therefore, a possible association between these polyomaviruses and PC was investigated. MATERIALS AND METHODS: Urine, blood and fresh prostatic tissue specimens were collected from 26 patients with PC. The presence of BKV and JCV, the possible non-coding control region (NCCR) variations and the genotyping analysis of viral protein 1 (VP1) of both viruses were assessed. RESULTS: Data showed a preferential viral re-activation in the urinary compartment and a statistically significant prevalence of JC viruria and of BKV in PC tissues. A BKV DDP-like NCCR sequence was isolated in two patients, whereas JCV NCCR was consistently of an archetypal structural organization. A prevalence of the European genotypes was observed for both viruses. CONCLUSION: Our data demonstrated the presence of JCV DNA in 14/24 (58.3%) cancerous prostatic tissue specimens, confirming the results obtained in a previous study, in which JCV has been defined as common inhabitant of the prostate, and opening the discussion about its potential role in PC. Copyright
Authors: Elena Anzivino; Maria Antonella Zingaropoli; Marco Iannetta; Valeria Antonietta Pietropaolo; Alessandra Oliva; Francesco Iori; Antonio Ciardi; Donatella Maria Rodio; Francesca Antonini; Cesare Giovanni Fedele; Alessandra D'Abramo; Claudio Maria Mastroianni; Vincenzo Vullo; Maria Rosa Ciardi Journal: Cancer Genomics Proteomics Date: 2016 11-12 Impact factor: 4.069