Fukang Teng1, Ying Yin2, Yajun Cui3, Yanping Deng2, Defang Li4, Kenka Cho5, Ge Zhang4, Aiping Lu4, Wanying Wu2, Min Yang2, Xuan Liu2, De-an Guo2, Jun Yin6, Baohong Jiang7. 1. Shenyang Pharmaceutical University, Wenhua Road #103, Shenyang 110016, China; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 3. Shanghai University of Traditional Chinese Medicine, Cailun Road #1200, Shanghai 201203, China. 4. Institute for Advancing Translational Medicine in Bone and Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China. 5. Takarazuka University of Medical and Health Care, Hanayashiki-Midorigaoka, Takarazuka City 6660162, Japan. 6. Shenyang Pharmaceutical University, Wenhua Road #103, Shenyang 110016, China. Electronic address: yinjun826@sina.com. 7. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. Electronic address: jiangbh@simm.ac.cn.
Abstract
AIMS: Despite the numerous pharmacological agents available for hypertension therapy, hypertension-related microvascular remodeling is not resolved, eventually leading to end-organ damage. The aim of the present study was to investigate the protection of salvianolic acid A (SalA) against microvascular remodeling in vitro and in vivo. MAIN METHODS: Spontaneously hypertensive rats (SHRs) were administered 2.5, 5 or 10 mg/kg SalA via intraperitoneal injection once a day for 4 weeks. The tail-cuff method was applied to monitor blood pressure; the microvascular structure of the retina was detected by hematoxylin-eosin and immunohistochemical staining; the function of mesenteric arteries was measured by DMT wire myography; endothelial cell proliferation was estimated using the Cell Counting Kit-8; endothelial cell migration was evaluated by wound healing and transwell assay; and endothelial cell integrity was detected by transendothelial electrical resistance and permeability assays. KEY FINDINGS: Although no antihypertensive effects of SalA were observed, SalA attenuated the microvascular inward remodeling of the retina and improved microvascular function in the mesenteries in vivo. Further cell experiments confirmed the beneficial effects of SalA on the integrity of the endothelial monolayer in vitro. SIGNIFICANCE: Salvianolic acid A inhibited endothelial dysfunction and vascular remodeling in spontaneously hypertensive rats. Therefore, salvianolic acid A could be a potential drug therapy to prevent further targeted organ damage induced by vascular remodeling.
AIMS: Despite the numerous pharmacological agents available for hypertension therapy, hypertension-related microvascular remodeling is not resolved, eventually leading to end-organ damage. The aim of the present study was to investigate the protection of salvianolic acid A (SalA) against microvascular remodeling in vitro and in vivo. MAIN METHODS: Spontaneously hypertensiverats (SHRs) were administered 2.5, 5 or 10 mg/kg SalA via intraperitoneal injection once a day for 4 weeks. The tail-cuff method was applied to monitor blood pressure; the microvascular structure of the retina was detected by hematoxylin-eosin and immunohistochemical staining; the function of mesenteric arteries was measured by DMT wire myography; endothelial cell proliferation was estimated using the Cell Counting Kit-8; endothelial cell migration was evaluated by wound healing and transwell assay; and endothelial cell integrity was detected by transendothelial electrical resistance and permeability assays. KEY FINDINGS: Although no antihypertensive effects of SalA were observed, SalA attenuated the microvascular inward remodeling of the retina and improved microvascular function in the mesenteries in vivo. Further cell experiments confirmed the beneficial effects of SalA on the integrity of the endothelial monolayer in vitro. SIGNIFICANCE: Salvianolic acid A inhibited endothelial dysfunction and vascular remodeling in spontaneously hypertensiverats. Therefore, salvianolic acid A could be a potential drug therapy to prevent further targeted organ damage induced by vascular remodeling.
Authors: Yang Liu; Yachen Xu; Zhenhua Wang; Dezhong Wen; Wentian Zhang; Sebastian Schmull; Haiyan Li; Yao Chen; Song Xue Journal: Am J Transl Res Date: 2016-04-15 Impact factor: 4.060