Martina Klemenak1, Jernej Dolinšek2, Tomaž Langerholc3, Diana Di Gioia4, Dušanka Mičetić-Turk5. 1. Department of Paediatrics, University Clinical Center Maribor, Ljubljanska ulica 5, 2000, Maribor, Slovenia. martina.klemenak@gmail.com. 2. Department of Paediatrics, University Clinical Center Maribor, Ljubljanska ulica 5, 2000, Maribor, Slovenia. jernej_dolinsek@hotmail.com. 3. Department of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, 2311, Hoče, Slovenia. tomaz.langerholc@um.si. 4. Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, 40127, Bologna, Italy. diana.digioia@unibo.it. 5. Department of Paediatrics, Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000, Maribor, Slovenia. dusanka.micetic@um.si.
Abstract
BACKGROUND: Increasing evidence suggests that not only genetics, but also environmental factors like gut microbiota dysbiosis play an important role in the pathogenesis of celiac disease (CD). AIM: The aim of our study was to investigate the effect of two probiotic strains Bifidobacterium breve BR03 and B. breve B632 on serum production of anti-inflammatory cytokine interleukin 10 (IL-10) and pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α) in children with CD. METHODS: The study was a double-blinded, placebo-controlled trial that included 49 children with CD on gluten-free diet (GFD) randomized into two groups and 18 healthy children in the control group. The first group (24 children with CD) daily received B. breve BR03 and B632 (2 × 10(9) colony-forming units) and the second group (25 children with CD) received placebo for 3 months. RESULTS:TNF-α levels were significantly decreased in the first group after receiving B. breve for 3 months. On follow-up, 3 months after receiving probiotics, TNF-α levels increased again. Children with CD who were on GFD for less than 1 year showed similar baseline TNF-α levels as children who were on GFD for more than 1 year. IL-10 levels were in all groups of patients below detection level. CONCLUSIONS:Probiotic intervention with B. breve strains has shown a positive effect on decreasing the production of pro-inflammatory cytokine TNF-α in children with CD on GFD.
RCT Entities:
BACKGROUND: Increasing evidence suggests that not only genetics, but also environmental factors like gut microbiota dysbiosis play an important role in the pathogenesis of celiac disease (CD). AIM: The aim of our study was to investigate the effect of two probiotic strains Bifidobacterium breve BR03 and B. breve B632 on serum production of anti-inflammatory cytokine interleukin 10 (IL-10) and pro-inflammatory cytokine tumornecrosis factor alpha (TNF-α) in children with CD. METHODS: The study was a double-blinded, placebo-controlled trial that included 49 children with CD on gluten-free diet (GFD) randomized into two groups and 18 healthy children in the control group. The first group (24 children with CD) daily received B. breve BR03 and B632 (2 × 10(9) colony-forming units) and the second group (25 children with CD) received placebo for 3 months. RESULTS: TNF-α levels were significantly decreased in the first group after receiving B. breve for 3 months. On follow-up, 3 months after receiving probiotics, TNF-α levels increased again. Children with CD who were on GFD for less than 1 year showed similar baseline TNF-α levels as children who were on GFD for more than 1 year. IL-10 levels were in all groups of patients below detection level. CONCLUSIONS: Probiotic intervention with B. breve strains has shown a positive effect on decreasing the production of pro-inflammatory cytokine TNF-α in children with CD on GFD.
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