Literature DB >> 26134133

Detection of Staphylococcus aureus enterotoxin production genes from patient samples using an automated extraction platform and multiplex real-time PCR.

Amy K Chiefari1, Michael J Perry1, Cassandra Kelly-Cirino1, Christina T Egan2.   

Abstract

To minimize specimen volume, handling and testing time, we have developed two TaqMan(®) multiplex real-time PCR (rtPCR) assays to detect staphylococcal enterotoxins A-E and Toxic Shock Syndrome Toxin production genes directly from clinical patient stool specimens utilizing a novel lysis extraction process in parallel with the Roche MagNA Pure Compact. These assays are specific, sensitive and reliable for the detection of the staphylococcal enterotoxin encoding genes and the tst1 gene from known toxin producing strains of Staphylococcus aureus. Specificity was determined by testing a total of 47 microorganism strains, including 8 previously characterized staphylococcal enterotoxin producing strains against each rtPCR target. Sensitivity for these assays range from 1 to 25 cfu per rtPCR reaction for cultured isolates and 8-20 cfu per rtPCR for the clinical stool matrix.
Copyright © 2015 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Automated extraction; Diagnostic testing; Multiplex PCR; Real-time PCR; Staphylococcus aureus

Mesh：

Substances：

Year:  2015        PMID： 26134133     DOI： 10.1016/j.mcp.2015.06.004

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


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