Hamit Kaan Müştak1, Elçin Günaydin2, İnci Başak Kaya1, Merve Özdal Salar1, Orkun Babacan1, Kaan Önat3, Zafer Ata4, Kadir Serdar Diker1. 1. a Department of Microbiology , Ankara University Faculty of Veterinary Medicine , Dışkapı , Ankara , Turkey. 2. b Breeding Disease Diagnosis Laboratory , Veterinary Control Central Research Institute , Etlik , Ankara , Turkey. 3. c Manyas Ministry of Food, Agriculture and Livestock, Republic of Turkey Ministry of Food , Agriculture and Livestock , Manyas , Bandırma , Turkey. 4. d Military Veterinary School and Educational Central Commandership , Gemlik , Bursa , Turkey.
Abstract
BACKGROUND: Escherichia coli is one of the major causative agents of bovine mastitis worldwide, and is typically associated with acute, clinical mastitis. Besides this, E. coli strains which belong to the extra-intestinal pathogenic group are also the major cause of urinary tract infections and pyometra in dogs. OBJECTIVES: In this study, it was aimed to investigate phylo-groups/subgroups in 155 E. coli isolates obtained from acute bovine mastitis, 43 from urinary tract infections of dogs and 20 from canine pyometra by a formerly described triplex PCR and recently described new quadruplex polymerase chain reaction (PCR) method. RESULTS: Group A1 (n = 118; 76%) and B1 (n = 71; 46%) were found to be the most prevalent groups by triplex and quadruplex PCR assays in mastitis isolates, respectively. Phylo-typing of 43 urinary tract isolates also revealed that most of the isolates belonged to A1 (n = 23; 54%) by triplex and B2 (n = 36; 84%) by quadruplex PCR assays. The isolates assigned as group A1 (n = 17; 85%) by triplex PCR could not be classified by quadruplex PCR in pyometra isolates. CONCLUSIONS: The results support the hypothesis that E. coli strains isolated from bovine mastitis cases are environmental. Also, groups C, E and F were identified as new phylo-groups for the first time in acute bovine mastitis cases. The comparison of triplex PCR with quadruplex PCR results revealed that most of the groups assigned in triplex PCR were altered by quadruplex PCR assay.
BACKGROUND:Escherichia coli is one of the major causative agents of bovinemastitis worldwide, and is typically associated with acute, clinical mastitis. Besides this, E. coli strains which belong to the extra-intestinal pathogenic group are also the major cause of urinary tract infections and pyometra in dogs. OBJECTIVES: In this study, it was aimed to investigate phylo-groups/subgroups in 155 E. coli isolates obtained from acute bovinemastitis, 43 from urinary tract infections of dogs and 20 from canine pyometra by a formerly described triplex PCR and recently described new quadruplex polymerase chain reaction (PCR) method. RESULTS: Group A1 (n = 118; 76%) and B1 (n = 71; 46%) were found to be the most prevalent groups by triplex and quadruplex PCR assays in mastitis isolates, respectively. Phylo-typing of 43 urinary tract isolates also revealed that most of the isolates belonged to A1 (n = 23; 54%) by triplex and B2 (n = 36; 84%) by quadruplex PCR assays. The isolates assigned as group A1 (n = 17; 85%) by triplex PCR could not be classified by quadruplex PCR in pyometra isolates. CONCLUSIONS: The results support the hypothesis that E. coli strains isolated from bovinemastitis cases are environmental. Also, groups C, E and F were identified as new phylo-groups for the first time in acute bovinemastitis cases. The comparison of triplex PCR with quadruplex PCR results revealed that most of the groups assigned in triplex PCR were altered by quadruplex PCR assay.
Authors: Rafael Gariglio Clark Xavier; Paloma Helena Sanches da Silva; Hanna Dornelas Trindade; Gabriela Muniz Carvalho; Rafael Romero Nicolino; Patrícia Maria Coletto Freitas; Rodrigo Otávio Silveira Silva Journal: Vet Sci Date: 2022-05-22