OBJECTIVE: Sufficient evidence suggests that the c-kit protooncogene receptor and its ligand stem cell factor (scf) signal pathway play a crucial role in interstitial cells of Cajal (ICCs) development and maintenance of their phenotype. We aimed to determine the expressions of scf mRNA and scf protein in the gallbladders in guinea pigs fed on high cholesterol diet (HCD). METHODS: The gallbladder and serum samples from 20 guinea pigs of HCD and from 20 guinea pigs of standard diet (StD) were used for this study. Serum lipid analysis was performed using standard laboratory procedure. Expression of scf mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR), and expression of scf protein was detected by Western blot analysis. RESULTS: Laboratory results showed serum total cholesterol (TC), low density lipoprotein (LDL) cholesterol , high density lipoprotein (HDL) cholesterol and triglyceride (TG) concentrations were significantly higher in the HCD group than in the StD group of guinea pigs (P < 0.001, respectively). Decreased expression of scf mRNA and protein were demonstrated in the HCD group compared with the StD group (P < 0.05 respectively). CONCLUSION: The data indicates that the expression of scf mRNA and c-kit protein is significantly decreased in the gallbladders in guinea pigs of HCD.
OBJECTIVE: Sufficient evidence suggests that the c-kit protooncogene receptor and its ligand stem cell factor (scf) signal pathway play a crucial role in interstitial cells of Cajal (ICCs) development and maintenance of their phenotype. We aimed to determine the expressions of scf mRNA and scf protein in the gallbladders in guinea pigs fed on high cholesterol diet (HCD). METHODS: The gallbladder and serum samples from 20 guinea pigs of HCD and from 20 guinea pigs of standard diet (StD) were used for this study. Serum lipid analysis was performed using standard laboratory procedure. Expression of scf mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR), and expression of scf protein was detected by Western blot analysis. RESULTS: Laboratory results showed serum total cholesterol (TC), low density lipoprotein (LDL) cholesterol , high density lipoprotein (HDL) cholesterol and triglyceride (TG) concentrations were significantly higher in the HCD group than in the StD group of guinea pigs (P < 0.001, respectively). Decreased expression of scf mRNA and protein were demonstrated in the HCD group compared with the StD group (P < 0.05 respectively). CONCLUSION: The data indicates that the expression of scf mRNA and c-kit protein is significantly decreased in the gallbladders in guinea pigs of HCD.
Authors: Artur Pasternak; Krzysztof Gil; Andrzej Matyja; Mariusz Gajda; Krystyna Sztefko; Jerzy A Walocha; Jan Kulig; Piotr Thor Journal: Neurogastroenterol Motil Date: 2012-11-01 Impact factor: 3.598
Authors: Artur Pasternak; Mariusz Gajda; Krzysztof Gil; Andrzej Matyja; Krzysztof A Tomaszewski; Jerzy A Walocha; Jan Kulig; Piotr Thor Journal: Folia Histochem Cytobiol Date: 2012 Impact factor: 1.698