Ying Zhang1, Baifeng Liu2, Jiang Shao3, Jia Song3, Jing Zhang3. 1. Department of Orthopedics, Changzheng Hospital Shanghai 200003, China. 2. Department of Orthopedics, Daqing Peopele's Hospital Daqing 163316, Heilongjiang Province, China. 3. Department of Orthopedic Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine 1665 Kongjiang Road, Shanghai 200092, China.
Abstract
OBJECTIVE: To identify putative biomarkers for ossification of posterior longitudinal ligament (OPLL). MATERIAL AND METHODS: Proteomic analysis was performed in 4 ligament samples from OPLL patients and healthy controls. RT-PCR was used to further verify the proteomic analysis results. RESULTS: A total of 50 differentially expressed spots were detected in 2-D electrophoresis between the two groups. In protein/peptide analysis, 21 proteins or peptides were finally identified. Besides 13 hematic proteins and 2 unknown proteins, 6 other proteins were differentially expressed. Among them, carbonic anhydrase I, NAD(P) dependent steroid dehydrogenase-like, billiverdin reductase B and alpha-1 collagen VI were down-regulated, while osteoglycin and nebulin-related anchoring protein were up-regulated. The results of NAD(P) dependent steroid dehydrogenase-like, alpha-1 collagen VI and nebulin-related anchoring protein were validated by RT-PCR. CONCLUSION: These differentially expressed proteins could play a role in the onset and progression of OPLL.
OBJECTIVE: To identify putative biomarkers for ossification of posterior longitudinal ligament (OPLL). MATERIAL AND METHODS: Proteomic analysis was performed in 4 ligament samples from OPLLpatients and healthy controls. RT-PCR was used to further verify the proteomic analysis results. RESULTS: A total of 50 differentially expressed spots were detected in 2-D electrophoresis between the two groups. In protein/peptide analysis, 21 proteins or peptides were finally identified. Besides 13 hematic proteins and 2 unknown proteins, 6 other proteins were differentially expressed. Among them, carbonic anhydrase I, NAD(P) dependent steroid dehydrogenase-like, billiverdin reductase B and alpha-1 collagen VI were down-regulated, while osteoglycin and nebulin-related anchoring protein were up-regulated. The results of NAD(P) dependent steroid dehydrogenase-like, alpha-1 collagen VI and nebulin-related anchoring protein were validated by RT-PCR. CONCLUSION: These differentially expressed proteins could play a role in the onset and progression of OPLL.
Entities:
Keywords:
2-dimensionl electrophoresis; Ossification of posterior longitudinal ligament; biomarker; diagnosis
Authors: M Rühl; M Johannsen; J Atkinson; D Manski; E Sahin; R Somasundaram; E O Riecken; D Schuppan Journal: Exp Cell Res Date: 1999-08-01 Impact factor: 3.905