Tao Ma1, Zhi-Qiang Ma2, Xiao-Hui Du2, Qiu-Shi Yu2, Rong Wang2, Li Liu2. 1. Hubei University of Medicine 30 South Renmin Road, Shiyan, Hubei 442000, China. 2. Department of Cardiology, 161 Hospital of PLA Wuhan 430010, China.
Abstract
OBJECTIVE: To study the effect of valsartan on ACAT-1 and PPAR-γ expression after vascular endothelial balloon injury in intimal hyperplasia process. METHODS: 24 male New Zealand white rabbits were randomly divided into three groups with 8 in each group. CONTROL GROUP: rabbits were fed with normal diet; Balloon injury group: rabbits were fed with 0.5% cholesterol, 5% lard rabbit feed; balloon injury + valsartan group, rabbits were fed with 0.5% cholesterol, 5% lard rabbit feed added with 10 mg/(kg.d) valsartan gavage. RT-PCR and Western blotting method were used to detect the carotid ACAT-1, PPAR-γ mRNA and protein expression after 8 weeks of feeding. RESULTS: In carotid artery balloon injury group, vascular smooth muscle cells (VSMC) proliferation and intimal hyperplasia were significantly higher 14 d after endothelial injury. In 14 days valsartan treatment group VSMC proliferation and intimal hyperplasia were lighter than the surgery group. Compared with the control group, ACAT-1, PPAR-γ mRNA and protein were significantly increased in balloon injury group and valsartan group (P < 0.01 or P < 0.05); the expression of ACAT-1 mRNA and protein were significantly lower in valsartan group and balloon injury group (P < 0.01 or P < 0.05). The expression of PPAR-γ mRNA and protein in valsartan group expression was significantly higher than that in the balloon injury group (P < 0.05). The expression level of ACAT-1 and PPAR-γ mRNA in balloon injury group and valsartan group showed negative correlation (P < 0.05). CONCLUSION: The expression of ACAT-1, PPAR-γ mRNA and protein content were significantly increased in intimal hyperplasia process after vascular endothelial balloon injury. The effect of valsartan suppressed intimal hyperplasia correlated with the expression of down-regulated ACAT-1 and up-regulated PPAR-γ.
OBJECTIVE: To study the effect of valsartan on ACAT-1 and PPAR-γ expression after vascular endothelial balloon injury in intimal hyperplasia process. METHODS: 24 male New Zealand white rabbits were randomly divided into three groups with 8 in each group. CONTROL GROUP: rabbits were fed with normal diet; Balloon injury group: rabbits were fed with 0.5% cholesterol, 5% lard rabbit feed; balloon injury + valsartan group, rabbits were fed with 0.5% cholesterol, 5% lard rabbit feed added with 10 mg/(kg.d) valsartan gavage. RT-PCR and Western blotting method were used to detect the carotid ACAT-1, PPAR-γ mRNA and protein expression after 8 weeks of feeding. RESULTS: In carotid artery balloon injury group, vascular smooth muscle cells (VSMC) proliferation and intimal hyperplasia were significantly higher 14 d after endothelial injury. In 14 days valsartan treatment group VSMC proliferation and intimal hyperplasia were lighter than the surgery group. Compared with the control group, ACAT-1, PPAR-γ mRNA and protein were significantly increased in balloon injury group and valsartan group (P < 0.01 or P < 0.05); the expression of ACAT-1 mRNA and protein were significantly lower in valsartan group and balloon injury group (P < 0.01 or P < 0.05). The expression of PPAR-γ mRNA and protein in valsartan group expression was significantly higher than that in the balloon injury group (P < 0.05). The expression level of ACAT-1 and PPAR-γ mRNA in balloon injury group and valsartan group showed negative correlation (P < 0.05). CONCLUSION: The expression of ACAT-1, PPAR-γ mRNA and protein content were significantly increased in intimal hyperplasia process after vascular endothelial balloon injury. The effect of valsartan suppressed intimal hyperplasia correlated with the expression of down-regulated ACAT-1 and up-regulated PPAR-γ.
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