Literature DB >> 2612893

Characterization of a methyl-specific restriction system in Clostridium acetobutylicum strain N1-4081.

H Azeddoug1, J Hubert, G Reysset.   

Abstract

A type II restriction endonuclease, named CacI, was detected in Clostridium acetobutylicum strain N1-4081. CacI cleaved the tetranucleotide sequence [5' decreases -GATC-3']. The modification system consisted of the methylation of the adenine present in this sequence. CacI, an isoschizomer of MboI, is inactive on dam methylated substrates.

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Year:  1989        PMID: 2612893     DOI: 10.1016/0378-1097(89)90239-5

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN: 0378-1097            Impact factor:   2.742


  3 in total

1.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971

2.  Restriction endonuclease activity in Clostridium thermocellum and Clostridium thermosaccharolyticum.

Authors:  T R Klapatch; A L Demain; L R Lynd
Journal:  Appl Microbiol Biotechnol       Date:  1996-03       Impact factor: 4.813

3.  Engineering clostridium strain to accept unmethylated DNA.

Authors:  Hongjun Dong; Yanping Zhang; Zongjie Dai; Yin Li
Journal:  PLoS One       Date:  2010-02-09       Impact factor: 3.240

  3 in total

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