Development and characterization of microsatellite markers via cross-species amplification of Paramisgurnus dabryanus.

The large-scale loach, Paramisgurnus dabryanus, is a small freshwater fish of major economic importance in many Asian countries, particularly China and South Korea. Fifteen polymorphic microsatellite (simple sequence repeat) markers were obtained through cross-species amplification between this loach and a related species, Misgurnus anguillicaudatus (GenBank accession numbers: KC117456 to KC117470). The number of alleles per locus ranged from 5 to 12 among 40 individuals, and the average observed and expected heterozygosities were 0.344 and 0.828, respectively. Three loci showed significant deviations from Hardy-Weinberg equilibrium. These polymorphic loci could provide a valuable tool for investigations of the population genetics, phylogeography, and conservation genetics of P. dabryanus.