Jae Ho Chung 1 , Seung Hwan Lee 2 , Chul Won Park 1 , Kyung Rae Kim 1 , Kyung Tae 1 , Sung-Ho Kang 3 , Young Ha Oh 4 , Ju Yeon Pyo 4 Show Affiliations »
Abstract
OBJECTIVES: To explore the role of antiapoptotic and apoptotic processes in the development of cholesteatoma by investigating the expression of an antiapoptotic (c-FLIP) and apoptotic (p53) protein relative to the expression of a proliferation marker (Ki-67). STUDY DESIGN: Basic science study. SETTING: Tertiary referral center. SUBJECTS AND METHODS: An immunohistochemical investigation was performed on 35 cholesteatoma specimens (21 acquired, 14 congenital) and 10 normal retroauricular skins to evaluate the expression of c-FLIP, p53, and Ki-67. The expression rate of each marker was measured to assess the difference between retroauricular skin and cholesteatoma, as well as between congenital and acquired cholesteatoma. RESULTS: c-FLIP expression was significantly higher in the cholesteatoma specimens than in retroauricular skin (P < .05), while the expression of p53 did not significantly differ between the two. Ki-67 expression in cholesteatoma was significantly higher than in retroauricular skin (P < .001). The c-FLIP expression rate was positively correlated with that of Ki-67 (r = 0.47, P = .001), and there was no significant correlation between the expression level of p53 and that of Ki-67 (r = 0.152, P = .319). In addition, no differences in c-FLIP, p53, and Ki-67 expression rates were evident between congenital and acquired cholesteatoma. CONCLUSIONS: The upregulation of c-FLIP together with unchanged p53 suggests an altered equilibrium between apoptosis and antiapoptosis, favoring antiapoptosis, and may play a role in the pathogenesis of cholesteatoma. © American Academy of Otolaryngology—Head and Neck Surgery Foundation 2015.
OBJECTIVES: To explore the role of antiapoptotic and apoptotic processes in the development of cholesteatoma by investigating the expression of an antiapoptotic (c-FLIP ) and apoptotic (p53 ) protein relative to the expression of a proliferation marker (Ki-67). STUDY DESIGN: Basic science study. SETTING: Tertiary referral center. SUBJECTS AND METHODS: An immunohistochemical investigation was performed on 35 cholesteatoma specimens (21 acquired, 14 congenital) and 10 normal retroauricular skins to evaluate the expression of c-FLIP , p53 , and Ki-67. The expression rate of each marker was measured to assess the difference between retroauricular skin and cholesteatoma , as well as between congenital and acquired cholesteatoma . RESULTS: c-FLIP expression was significantly higher in the cholesteatoma specimens than in retroauricular skin (P < .05), while the expression of p53 did not significantly differ between the two. Ki-67 expression in cholesteatoma was significantly higher than in retroauricular skin (P < .001). The c-FLIP expression rate was positively correlated with that of Ki-67 (r = 0.47, P = .001), and there was no significant correlation between the expression level of p53 and that of Ki-67 (r = 0.152, P = .319). In addition, no differences in c-FLIP , p53 , and Ki-67 expression rates were evident between congenital and acquired cholesteatoma . CONCLUSIONS: The upregulation of c-FLIP together with unchanged p53 suggests an altered equilibrium between apoptosis and antiapoptosis, favoring antiapoptosis, and may play a role in the pathogenesis of cholesteatoma . © American Academy of Otolaryngology—Head and Neck Surgery Foundation 2015.
Entities: Disease
Gene
Keywords:
Ki-67; apoptosis; c-FLIP; cholesteatoma; p53
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Year: 2015
PMID： 26124267 DOI： 10.1177/0194599815591810
Source DB: PubMed Journal: Otolaryngol Head Neck Surg ISSN： 0194-5998 Impact factor: 3.497