Wenkai Yu1, Xiaopeng Yuan2, Xingche Xu2, Rui Ding2, Liyuan Pang2, Yinhui Liu2, Yanjie Guo2, Huajun Li2, Ming Li2, Jieli Yuan2, Li Tang2, Shu Wen3. 1. Department of Microecology, College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning, China; Department of Laboratory, Jining No 1 People's Hospital, Jining, Shangdong, China. 2. Department of Microecology, College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning, China. 3. Department of Microecology, College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning, China. Electronic address: wsh2008cn@sina.com.
Abstract
BACKGROUND: An increased prevalence of allergic disorders in developed countries has been associated with decreased exposure to environmental micro-organisms and an alteration of microbiota colonization. An appropriate model is needed to investigate the mechanisms by which hygiene environment-driven changes in microbiota could regulate allergic disorders. OBJECTIVE: To discover the correlation between the higher incidence and severity of allergies with the relative hygiene environment in a developed country. METHODS: Allergic respiratory inflammation was induced in specific pathogen-free and control rats by sensitization and challenge with ovalbumin. The diversity of lower airway bacteria community was analyzed by polymerase chain reaction denaturing gradient gel electrophoresis and sequencing before ovalbumin sensitization. Allergic respiratory inflammation resulting in cellular infiltrate was measured after the last challenge. RESULTS: The diversity of microbiota in the airway of specific pathogen-free rats decreased compared with the control rats; the more frequent microbiota in the control rats were Proteobacteria and Bacteroidetes. In addition, increased nasal rubbing and sneezing combined with exaggerated IgE production and leukocyte number was observed in ovalbumin-treated specific pathogen-free rats. CONCLUSION: These data indicate that the excessive "hygienic" environment resulted in a decreased bacterial diversity in the airway during infancy, leading to an increased susceptibility to allergic disease.
BACKGROUND: An increased prevalence of allergic disorders in developed countries has been associated with decreased exposure to environmental micro-organisms and an alteration of microbiota colonization. An appropriate model is needed to investigate the mechanisms by which hygiene environment-driven changes in microbiota could regulate allergic disorders. OBJECTIVE: To discover the correlation between the higher incidence and severity of allergies with the relative hygiene environment in a developed country. METHODS:Allergic respiratory inflammation was induced in specific pathogen-free and control rats by sensitization and challenge with ovalbumin. The diversity of lower airway bacteria community was analyzed by polymerase chain reaction denaturing gradient gel electrophoresis and sequencing before ovalbumin sensitization. Allergic respiratory inflammation resulting in cellular infiltrate was measured after the last challenge. RESULTS: The diversity of microbiota in the airway of specific pathogen-free rats decreased compared with the control rats; the more frequent microbiota in the control rats were Proteobacteria and Bacteroidetes. In addition, increased nasal rubbing and sneezing combined with exaggerated IgE production and leukocyte number was observed in ovalbumin-treated specific pathogen-free rats. CONCLUSION: These data indicate that the excessive "hygienic" environment resulted in a decreased bacterial diversity in the airway during infancy, leading to an increased susceptibility to allergic disease.
Authors: Benjamin A Turturice; Ravi Ranjan; Brian Nguyen; Lauren M Hughes; Kalista E Andropolis; Diane R Gold; Augusto A Litonjua; Emily Oken; David L Perkins; Patricia W Finn Journal: Am J Respir Cell Mol Biol Date: 2017-10 Impact factor: 6.914
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