The human genes for calbindin 27 and 29 kDa proteins are located on chromosomes 8 and 16, respectively.

Genomic clones coding for the brain calcium-binding protein, calbindin 29 kDa, were isolated from a human library. A fragment containing exon 2 was used as a probe to investigate the presence of the gene in human x rodent somatic cell hybrids. The gene was unambiguously assigned to chromosome 16. The closely-related calbindin 27 kDa gene was previously assigned to chromosome 8. These two genes, deriving from a common ancestor, thus appear to have been separated during vertebrate evolution.