Carla Rodrigues1, Elisabete Machado1,2, João Pires1, Helena Ramos3, Ângela Novais1, Luísa Peixe1. 1. UCIBIO/REQUIMTE, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal. 2. CEBIMED/FP-ENAS, Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Porto, Portugal. 3. Centro Hospitalar do Porto - Hospital de Santo António, Porto, Portugal.
Abstract
AIM: To characterize temporal shifts in extended-spectrum β-lactamases (ESBLs) and clones of clinical Escherichia coli isolates. MATERIALS & METHODS: All ESBL-producing E. coli isolates from a Portuguese hospital (n = 112; June 2006-June 2007 and January-December 2010) were characterized by identification of phylogenetic groups, ESBL-types and virulence genes, XbaI-PFGE and MLST. RESULTS: We observed a substantial increase in widespread E. coli clones from phylogroups A, B1 and D (e.g., ST10, ST23, ST117, ST155, ST648) producing mainly CTX-M-1, -14, -32 or SHV-12, along with a decrease in the proportion of the predominant CTX-M-15-producing B2-ST131 clone. CONCLUSION: The amplification of diverse CTX-M-producing A, B1 and D clonal complexes, which have been long identified in Portuguese nonclinical settings, unveils a role for these reservoirs in the landscape of ESBL-producing E. coli in the clinical setting.
AIM: To characterize temporal shifts in extended-spectrum β-lactamases (ESBLs) and clones of clinical Escherichia coli isolates. MATERIALS & METHODS: All ESBL-producing E. coli isolates from a Portuguese hospital (n = 112; June 2006-June 2007 and January-December 2010) were characterized by identification of phylogenetic groups, ESBL-types and virulence genes, XbaI-PFGE and MLST. RESULTS: We observed a substantial increase in widespread E. coli clones from phylogroups A, B1 and D (e.g., ST10, ST23, ST117, ST155, ST648) producing mainly CTX-M-1, -14, -32 or SHV-12, along with a decrease in the proportion of the predominant CTX-M-15-producing B2-ST131 clone. CONCLUSION: The amplification of diverse CTX-M-producing A, B1 and D clonal complexes, which have been long identified in Portuguese nonclinical settings, unveils a role for these reservoirs in the landscape of ESBL-producing E. coli in the clinical setting.
Authors: Sara A Ochoa; Ariadnna Cruz-Córdova; Victor M Luna-Pineda; Juan P Reyes-Grajeda; Vicenta Cázares-Domínguez; Gerardo Escalona; Ma Eugenia Sepúlveda-González; Fernanda López-Montiel; José Arellano-Galindo; Briceida López-Martínez; Israel Parra-Ortega; Silvia Giono-Cerezo; Rigoberto Hernández-Castro; Daniela de la Rosa-Zamboni; Juan Xicohtencatl-Cortes Journal: Front Microbiol Date: 2016-12-21 Impact factor: 5.640
Authors: Ana C Mendes; Carla Rodrigues; João Pires; José Amorim; Maria Helena Ramos; Ângela Novais; Luísa Peixe Journal: Emerg Infect Dis Date: 2016-02 Impact factor: 6.883
Authors: João Pires; Esther Kuenzli; Sara Kasraian; Regula Tinguely; Hansjakob Furrer; Markus Hilty; Christoph Hatz; Andrea Endimiani Journal: Front Microbiol Date: 2016-07-12 Impact factor: 5.640