Jin-Shu Shi1, Juan Zhang1, Qiong Wu1, Jian Li2. 1. Department of Hematology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China. 2. Department of Hematology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China. E-mail: jeniferjian@gmail.com.
Abstract
OBJECTIVE: To investigate the value of CD64 mean fluorescence intensity of the peripheral blood neutrophils as a diagnostic marker of bacterial infection in patients with hematologic malignancies after chemotherapy. METHODS: The neutrophil CD64 mean fluorescence intensity of all patients was detected by flow cytometry, and compared with procalcitonin (PCT) and C reactive protein (CRP) detected in part of patients; the relationship between nCD64 and bacterial infection were analyzed through continuous dynamic monitoring nCD64 mean fluorescence intensity in part of patients. RESULTS: The expression of nCD64 was not affected by neutrophils counts (P>0.01); the nCD64 mean fluorescence intensity, PCT and CRP levels in infection group and dynamic monitoring group were significantly higher than those in non-infected group (P<0.01); the sensitivity and specificity of nCD64 mean fluorescence intensity were much higher, as compared with PCT and CRP in diagnosis of bacterial infection. CONCLUSION: nCD64 mean fluorescence intensity can be used as an effective diagnostic marker for bacterial infection in patients with hematologic malignancies after chemotherapy, and may be used to forecast bacterial infection to a certain extent.
OBJECTIVE: To investigate the value of CD64 mean fluorescence intensity of the peripheral blood neutrophils as a diagnostic marker of bacterial infection in patients with hematologic malignancies after chemotherapy. METHODS: The neutrophil CD64 mean fluorescence intensity of all patients was detected by flow cytometry, and compared with procalcitonin (PCT) and C reactive protein (CRP) detected in part of patients; the relationship between nCD64 and bacterial infection were analyzed through continuous dynamic monitoring nCD64 mean fluorescence intensity in part of patients. RESULTS: The expression of nCD64 was not affected by neutrophils counts (P>0.01); the nCD64 mean fluorescence intensity, PCT and CRP levels in infection group and dynamic monitoring group were significantly higher than those in non-infected group (P<0.01); the sensitivity and specificity of nCD64 mean fluorescence intensity were much higher, as compared with PCT and CRP in diagnosis of bacterial infection. CONCLUSION: nCD64 mean fluorescence intensity can be used as an effective diagnostic marker for bacterial infection in patients with hematologic malignancies after chemotherapy, and may be used to forecast bacterial infection to a certain extent.