Xin Zhang1, Yuan Deng1, Feng-Li Chen1, Yi-Jia Fang1, Wei Zhang2, Liang Yu3. 1. Department of Hematology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, Jiangsu Province, China. 2. Provincial Institute for Food and Drug Control, Huai'an 223300, Jiangsu Province, China. 3. Department of Hematology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, Jiangsu Province, China. E-mail: yuliangha@163.com.
Abstract
OBJECTIVE: To establish human B-acute lymphoblastic leukemia NOD/SCID mouse model. METHODS: The unirradiated mice aged 4-5 weeks were injected with Nalm-6 cells via the tail vein. Successful transplantation was assessed by the general state, bone marrow smear and histopathologic examination. RESULTS: After 15 days of injection with Nalm-6 cells, the NOD/SCID mice displayed the morbidily with lethargy, hind limb paralysis, hunched back and lossing weight. The infiltration of leukemia cells could be observed in bone marrow and spleen sections. CONCLUSION: Systemic B lineage acute leukemia animal mode1 has been successfully established in the NOD/SCID mice, which is a useful tool for studying pathogenesis of leukemia and guiding clinical chemotherapy regimens.
OBJECTIVE: To establish human B-acute lymphoblastic leukemia NOD/SCIDmouse model. METHODS: The unirradiated mice aged 4-5 weeks were injected with Nalm-6 cells via the tail vein. Successful transplantation was assessed by the general state, bone marrow smear and histopathologic examination. RESULTS: After 15 days of injection with Nalm-6 cells, the NOD/SCIDmice displayed the morbidily with lethargy, hind limb paralysis, hunched back and lossing weight. The infiltration of leukemia cells could be observed in bone marrow and spleen sections. CONCLUSION: Systemic B lineage acute leukemia animal mode1 has been successfully established in the NOD/SCIDmice, which is a useful tool for studying pathogenesis of leukemia and guiding clinical chemotherapy regimens.